MicroRNA-155 regulates monocyte chemokine and chemokine receptor expression in Rheumatoid Arthritis

To test the hypothesis that miR-155 regulates monocyte migratory potential via modulation of chemokine and chemokine receptor expression in RA, and thereby is associated with disease activity. The miR-155 copy-numbers in monocytes from peripheral blood (PB) of healthy (n = 22), RA (n = 24) and RA SF...

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Published in:Rheumatology (Oxford, England) England), 2016-11, Vol.55 (11), p.2056-2065
Main Authors: Elmesmari, Aziza, Fraser, Alasdair R, Wood, Claire, Gilchrist, Derek, Vaughan, Diane, Stewart, Lynn, McSharry, Charles, McInnes, Iain B, Kurowska-Stolarska, Mariola
Format: Article
Language:English
Subjects:
Adult
Aged
Arthritis, Rheumatoid - genetics
Arthritis, Rheumatoid - metabolism
Basic Science
Cells, Cultured
Chemokines - biosynthesis
Chemokines - metabolism
Cytokines - biosynthesis
Cytokines - metabolism
Down-Regulation
Epigenesis, Genetic - genetics
Female
Humans
Leukocytes, Mononuclear - metabolism
Lipopolysaccharide Receptors - metabolism
Male
MicroRNAs - metabolism
MicroRNAs - physiology
Middle Aged
Receptors, Chemokine - metabolism
Synovial Membrane - metabolism
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Summary:To test the hypothesis that miR-155 regulates monocyte migratory potential via modulation of chemokine and chemokine receptor expression in RA, and thereby is associated with disease activity. The miR-155 copy-numbers in monocytes from peripheral blood (PB) of healthy (n = 22), RA (n = 24) and RA SF (n = 11) were assessed by real time-PCR using synthetic miR-155 as a quantitative standard. To evaluate the functional impact of miR-155, human monocytes were transfected with control or miR-155 mimic, and the effect on transcript levels, and production of chemokines was evaluated by Taqman low-density arrays and multiplex assays. A comparative study evaluated constitutive chemokine receptor expression in miR-155 and wild-type murine (CD115   Ly6C   Ly6G ) monocytes. Compared with healthy monocytes, the miR-155 copy-number was higher in RA, peripheral blood (PB) and SF monocytes (PB P 
ISSN:1462-0324
1462-0332
DOI:10.1093/rheumatology/kew272