Intravascular Delivery of Biologics to the Rat Kidney

The renal microvascular compartment plays an important role in the progression of kidney disease and hypertension, leading to the development of End Stage Renal Disease with high risk of death for cardiovascular events. Moreover, recent clinical studies have shown that renovascular structure and fun...

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Bibliographic Details
Published in:Journal of visualized experiments 2016-09 (115)
Main Authors: Franchi, Federico, Zhu, Xiang Yang, Witt, Tyra A, Lerman, Lilach O, Rodriguez-Porcel, Martin
Format: Article
Language:English
Subjects:
Animals
Biological Products - administration & dosage
Biological Products - pharmacokinetics
Catheterization - methods
Disease Models, Animal
Female
Kidney - blood supply
Kidney - drug effects
Kidney - metabolism
Kidney Diseases - drug therapy
Kidney Diseases - metabolism
Medicine
Rats
Rats, Sprague-Dawley
Recombinant Proteins - administration & dosage
Recombinant Proteins - pharmacokinetics
Vascular Endothelial Growth Factor A - administration & dosage
Vascular Endothelial Growth Factor A - pharmacokinetics
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Summary:The renal microvascular compartment plays an important role in the progression of kidney disease and hypertension, leading to the development of End Stage Renal Disease with high risk of death for cardiovascular events. Moreover, recent clinical studies have shown that renovascular structure and function may have a great impact on functional renal recovery after surgery. Here, we describe a protocol for the delivery of drugs into the renal artery of rats. This procedure offers significant advantages over the frequently used systemic administration as it may allow a more localized therapeutic effect. In addition, the use of rodents in pharmacodynamic analysis of preclinical studies may be cost effective, paving the way for the design of translational experiments in larger animal models. Using this technique, infusion of rat recombinant Vascular Endothelial Growth Factor (VEGF) protein in rats has induced activation of VEGF signaling as shown by increased expression of FLK1, pAKT/AKT, pERK/ERK. In summary, we established a protocol for the intrarenal delivery of drugs in rats, which is simple and highly reproducible.
ISSN:1940-087X
1940-087X
DOI:10.3791/54418