Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Stimuli-sensitive poly(N-isopropylacrylamide) (PNIPAM) microgels have various prospective practical applications and uses in fundamental research. In this work, we use single particle tracking of fluorescently labeled PNIPAM microgels as a showcase for tuning microgel size by a rapid non-stirred pre...

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Bibliographic Details
Published in:Journal of visualized experiments 2016-09 (115)
Main Authors: Virtanen, Otto L J, Purohit, Ashvini, Brugnoni, Monia, Wöll, Dominik, Richtering, Walter
Format: Article
Language:English
Subjects:
Acrylic Resins
Chemistry
Gels
Microscopy, Fluorescence
Particle Size
Polymerization
Prospective Studies
Temperature
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Summary:Stimuli-sensitive poly(N-isopropylacrylamide) (PNIPAM) microgels have various prospective practical applications and uses in fundamental research. In this work, we use single particle tracking of fluorescently labeled PNIPAM microgels as a showcase for tuning microgel size by a rapid non-stirred precipitation polymerization procedure. This approach is well suited for prototyping new reaction compositions and conditions or for applications that do not require large amounts of product. Microgel synthesis, particle size and structure determination by dynamic and static light scattering are detailed in the protocol. It is shown that the addition of functional comonomers can have a large influence on the particle nucleation and structure. Single particle tracking by wide-field fluorescence microscopy allows for an investigation of the diffusion of labeled tracer microgels in a concentrated matrix of non-labeled microgels, a system not easily investigated by other methods such as dynamic light scattering.
ISSN:1940-087X
1940-087X
DOI:10.3791/54419