Neutrophil expression of glucocorticoid-induced leucine zipper (GILZ) anti-inflammatory protein is associated with acute respiratory distress syndrome severity

• Published in: Annals of intensive care 2016-11, Vol.6 (1), p.105-105, Article 105
• Main Authors: Espinasse, Marie-Alix, Hajage, David, Montravers, Philippe, Piednoir, Pascale, Dufour, Guillaume, Tubach, Florence, Granger, Vanessa, de Chaisemartin, Luc, Noël, Benoît, Pallardy, Marc, Chollet-Martin, Sylvie, Biola-Vidamment, Armelle
• Format: Article
• Language: English
• Subjects: Anesthesiology; Critical Care Medicine; Emergency Medicine; Intensive; Intensive care; Life Sciences; Medicine; Medicine & Public Health
• ISSN: 2110-5820; 2110-5820
• DOI: 10.1186/s13613-016-0210-0

Background Glucocorticoid-induced leucine zipper (GILZ) is a potent anti-inflammatory protein involved in neutrophil apoptosis and the resolution of inflammation. Given the numerous pathophysiologic roles of neutrophils in the acute respiratory distress syndrome (ARDS), we postulated that neutrophil GILZ expression might be induced during ARDS, to modulate the inflammatory process and participate in lung repair. Methods This single-center, prospective, observational cohort study took place in the surgical intensive care unit of Bichat Hospital (Paris, France) and involved 17 ARDS patients meeting the Berlin criteria at inclusion, and 14 ventilated controls without ARDS. Serial blood samples were obtained every 2 days until extubation or death (from 1 to 9 samples per patient). GILZ protein and gene expression was quantified in blood neutrophils, along with markers of inflammation (CRP, extracellular DNA) or its resolution (Annexin A1). Results Neutrophil GILZ expression was detected at the transcriptional and/or translational level in 9/17 ARDS patients (in particular 7/10 severe ARDS) and in 2/14 ventilated controls. The highest mRNA levels were observed in the most severely ill patients ( p