A Nucleoporin Docks Protein Phosphatase 1 to Direct Meiotic Chromosome Segregation and Nuclear Assembly

• Published in: Developmental cell 2016-09, Vol.38 (5), p.463-477
• Main Authors: Hattersley, Neil, Cheerambathur, Dhanya, Moyle, Mark, Stefanutti, Marine, Richardson, Amelia, Lee, Kian-Yong, Dumont, Julien, Oegema, Karen, Desai, Arshad
• Format: Article
• Language: English
• Subjects: Animals; Caenorhabditis elegans - genetics; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cellular Biology; centromere; chromosome segregation; Chromosome Segregation - genetics; Development Biology; ELYS; Humans; kinetochore; Kinetochores - metabolism; Life Sciences; meiosis; Meiosis - genetics; MEL-28; Nuclear Envelope - genetics; Nuclear Envelope - metabolism; nuclear pore; nuclear pore assembly; Nuclear Pore Complex Proteins - genetics; Nuclear Pore Complex Proteins - metabolism; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Oocytes - growth & development; Oocytes - metabolism; PP1 docking; Protein Interaction Maps - genetics; protein phosphatase 1; Receptors, Neuropeptide Y - genetics; Receptors, Neuropeptide Y - metabolism
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2016.08.006

During M-phase entry in metazoans with open mitosis, the concerted action of mitotic kinases disassembles nuclei and promotes assembly of kinetochores—the primary microtubule attachment sites on chromosomes. At M-phase exit, these major changes in cellular architecture must be reversed. Here, we show that the conserved kinetochore-localized nucleoporin MEL-28/ELYS docks the catalytic subunit of protein phosphatase 1 (PP1c) to direct kinetochore disassembly-dependent chromosome segregation during oocyte meiosis I and nuclear assembly during the transition from M phase to interphase. During oocyte meiosis I, MEL-28-PP1c disassembles kinetochores in a timely manner to promote elongation of the acentrosomal spindles that segregate homologous chromosomes. During nuclear assembly, MEL-28 recruits PP1c to the periphery of decondensed chromatin, where it directs formation of a functional nuclear compartment. Thus, a pool of phosphatase activity associated with a kinetochore-localized nucleoporin contributes to two key events that occur during M-phase exit in metazoans: kinetochore disassembly and nuclear reassembly. [Display omitted] •The Y-complex nucleoporin MEL-28/ELYS is essential for meiosis I anaphase•MEL-28 acts in meiosis I anaphase by docking protein phosphatase I (PP1c)•MEL-28-PP1c promotes kinetochore disassembly-dependent segregation•MEL-28-PP1c directs nuclear assembly and expansion during M-phase exit A subset of nucleoporins transitions from nuclear pores in interphase to the centromere/kinetochore region of chromosomes in M phase. Hattersley et al. show that the kinetochore-localized nucleoporin MEL-28/ELYS docks the catalytic subunit of protein phosphatase 1 (PP1c) to direct two events: meiotic chromosome segregation and nuclear reassembly during M-phase exit.