Combined Elemental and Biomolecular Mass Spectrometry Imaging for Probing the Inventory of Tissue at a Micrometer Scale

Several complementary mass spectrometric imaging techniques allow mapping of various analytes within biological tissue sections. Laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) quantitatively detects elements and isotopes with very high sensitivity and a particularly high dyna...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-04, Vol.84 (7), p.3170-3178
Main Authors: Matusch, Andreas, Fenn, Larissa S, Depboylu, Candan, Klietz, Martin, Strohmer, Sven, McLean, John A, Becker, J. Sabine
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Lasers
Lipids
Male
Mass spectrometry
Mass Spectrometry - methods
Mice
Microtechnology - methods
Molecular Imaging - methods
Molecules
Optical Phenomena
Oxidopamine - pharmacology
Prosencephalon - drug effects
Prosencephalon - metabolism
Tissues
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Several complementary mass spectrometric imaging techniques allow mapping of various analytes within biological tissue sections. Laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) quantitatively detects elements and isotopes with very high sensitivity and a particularly high dynamical range. Matrix-assisted laser desorption/ionization ion mobility mass spectrometry (MALDI-IM-MS) allows a pixel-by-pixel classification and identification of biomolecules. In order to dispose of the healthy hemisphere as an internal calibrant in addition to routinely used external standards, adjacent brain sections of mice with a unilateral 6-OHDA lesion of the medial forebrain bundle were chosen as exemplary samples. We demonstrate a comprehensive way of data acquisition and analysis by coregistering mass spectrometric data on photomicrographs as common reference space and thus providing trimodal spatial information. Registering subsequent planar element maps yielded continuous 3-dimensional data sets. Furthermore, we introduce a correction of MSI data for variable slice thickness applicable to all MSI techniques. In the present case, we observed increased concentrations of iron, manganese, and copper in the lesioned substantia nigra while monounsaturated lipid levels were decreased in the identical region of interest. Our techniques provide new insights into the intricate spatial relationship of morphology and chemistry within tissue.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac203112c