Surface biotinylation of cytotoxic T lymphocytes for in vivo tracking of tumor immunotherapy in murine models

Currently, there is no stable and flexible method to label and track cytotoxic T lymphocytes (CTLs) in vivo in CTL immunotherapy. We aimed to evaluate whether the sulfo-hydroxysuccinimide (NHS)-biotin–streptavidin (SA) platform could chemically modify the cell surface of CTLs for in vivo tracking. C...

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Bibliographic Details
Published in:Cancer Immunology, Immunotherapy Immunotherapy, 2016-12, Vol.65 (12), p.1545-1554
Main Authors: Li, Anning, Wu, Yue, Linnoila, Jenny, Pulli, Benjamin, Wang, Cuihua, Zeller, Matthias, Ali, Muhammad, Lewandrowski, Grant K., Li, Jinghui, Tricot, Benoit, Keliher, Edmund, Wojtkiewicz, Gregory R., Fulci, Giulia, Feng, Xiaoyuan, Tannous, Bakhos A., Yao, Zhenwei, Chen, John W.
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Biotinylation - methods
Brain cancer
Brain research
Cancer Research
Chromatography
Cytotoxicity
Disease Models, Animal
Female
Humans
Immunology
Immunotherapy
Immunotherapy - methods
Labeling
Lymphocytes
Medicine
Medicine & Public Health
Mice
Oncology
Original Article
Peptides
T-Lymphocytes, Cytotoxic - metabolism
Tomography
Tumors
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Summary:Currently, there is no stable and flexible method to label and track cytotoxic T lymphocytes (CTLs) in vivo in CTL immunotherapy. We aimed to evaluate whether the sulfo-hydroxysuccinimide (NHS)-biotin–streptavidin (SA) platform could chemically modify the cell surface of CTLs for in vivo tracking. CD8+ T lymphocytes were labeled with sulfo-NHS-biotin under different conditions and then incubated with SA–Alexa647. Labeling efficiency was proportional to sulfo-NHS-biotin concentration. CD8+ T lymphocytes could be labeled with higher efficiency with sulfo-NHS-biotin in DPBS than in RPMI ( P  
ISSN:0340-7004
1432-0851
DOI:10.1007/s00262-016-1911-9