RNA interference mediated downregulation of human telomerase reverse transcriptase (hTERT) in LN18 cells

Human telomerase reverse transcriptase (hTERT) gene is a biomarker for the targeted therapy in various cancers. Presence of increased telomerase activity is a common feature of all cancers including glioblastoma. Both RNA and catalytic subunits of hTERT are the target sites for blocking its activity...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2016-12, Vol.68 (6), p.2311-2321
Main Authors: Lavanya, Ch, Sibin, M. K., Srinivas Bharath, M. M., Manoj, M. Jeru, Venkataswamy, Manjunatha M., Bhat, Dhananjaya I., Narasinga Rao, K. V. L., Chetan, G. K.
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Biomarkers
Biomedicine
Biotechnology
Brain cancer
Brain tumors
Carcinogenesis
Catalytic subunits
Cell death
Cell viability
Chemistry
Chemistry and Materials Science
Chromosomes
Down-regulation
Flow cytometry
Gene expression
Glioblastoma
Glioma
Immunoblotting
Laboratories
Medical prognosis
Original
Original Article
Patients
RNA polymerase
RNA-directed DNA polymerase
RNA-mediated interference
siRNA
Stem cells
Telomerase
Telomerase reverse transcriptase
Tumors
Yeast
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Summary:Human telomerase reverse transcriptase (hTERT) gene is a biomarker for the targeted therapy in various cancers. Presence of increased telomerase activity is a common feature of all cancers including glioblastoma. Both RNA and catalytic subunits of hTERT are the target sites for blocking its activity. The current study focuses on the expression of hTERT in glioblastoma and its regulation using two different novel siRNAs (small interfering RNA). Our patient data demonstrated increased expression of hTERT, which could be correlated with carcinogenesis in glioma. In vitro studies in siRNA transfected LN18 cells confirmed significant cell death ( p  
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-016-0025-8