Functional properties of in vitro excitatory cortical neurons derived from human pluripotent stem cells

The in vitro derivation of regionally defined human neuron types from patient‐derived stem cells is now established as a resource to investigate human development and disease. Characterization of such neurons initially focused on the expression of developmentally regulated transcription factors and...

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Bibliographic Details
Published in:The Journal of physiology 2016-11, Vol.594 (22), p.6573-6582
Main Authors: Livesey, Matthew R., Magnani, Dario, Hardingham, Giles E., Chandran, Siddharthan, Wyllie, David J. A.
Format: Article
Language:English
Subjects:
Action Potentials - physiology
Animals
Cell Differentiation - physiology
Cellular and Molecular Neuroscience
Humans
Neurons
Pluripotent Stem Cells - metabolism
Pluripotent Stem Cells - physiology
Stem cells
Symposium Review
Symposium section reviews: Are stem cell‐derived neural cells physiologically credible?
Transcription Factors - metabolism
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Summary:The in vitro derivation of regionally defined human neuron types from patient‐derived stem cells is now established as a resource to investigate human development and disease. Characterization of such neurons initially focused on the expression of developmentally regulated transcription factors and neural markers, in conjunction with the development of protocols to direct and chart the fate of differentiated neurons. However, crucial to the understanding and exploitation of this technology is to determine the degree to which neurons recapitulate the key functional features exhibited by their native counterparts, essential for determining their usefulness in modelling human physiology and disease in vitro. Here, we review the emerging data concerning functional properties of human pluripotent stem cell‐derived excitatory cortical neurons, in the context of both maturation and regional specificity. Many protocols now exist to derive excitatory cortical neurons from human pluripotent stem cells however the physiological properties of the neurons remain under explored.
ISSN:0022-3751
1469-7793
DOI:10.1113/JP270660