Prognostic value of 5-microRNA based signature in T2-T3N0 colon cancer

The role of adjuvant chemotherapy in stage T2-T3N0 colon cancer (CC) is controversial and there are currently no reliable factors allowing for individual selection of patients with high risk of relapse for such therapy. We searched for microRNA-based signature with prognostic significance in this gr...

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Bibliographic Details
Published in:Clinical & experimental metastasis 2016-12, Vol.33 (8), p.765-773
Main Authors: Bobowicz, Maciej, Skrzypski, Marcin, Czapiewski, Piotr, Marczyk, Michał, Maciejewska, Agnieszka, Jankowski, Michał, Szulgo-Paczkowska, Anna, Zegarski, Wojciech, Pawłowski, Ryszard, Polańska, Joanna, Biernat, Wojciech, Jaśkiewicz, Janusz, Jassem, Jacek
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Biomedical and Life Sciences
Biomedicine
Cancer Research
Colonic Neoplasms - genetics
Colonic Neoplasms - pathology
DNA Mismatch Repair - genetics
Female
Gene Expression Regulation, Neoplastic
Hematology
Humans
Kaplan-Meier Estimate
Male
MicroRNAs - biosynthesis
MicroRNAs - genetics
Middle Aged
Neoplasm Metastasis - genetics
Neoplasm Metastasis - pathology
Oncology
Predictive Value of Tests
Prognosis
Research Paper
Surgical Oncology
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Summary:The role of adjuvant chemotherapy in stage T2-T3N0 colon cancer (CC) is controversial and there are currently no reliable factors allowing for individual selection of patients with high risk of relapse for such therapy. We searched for microRNA-based signature with prognostic significance in this group. We assessed by qRT-PCR expression of 754 microRNAs (miRNAs) in tumour samples from 85 stage pT2-3N0 CC patients treated with surgery alone. MiRNA expression was compared between two groups of patients: 40 and 45 patients who did and did not develop distant metastases after resection, respectively. Additionally, miRNA expression was compared between CC and normal colon mucosa samples and between the mismatch repair (MMR) competent and deficient tumours. Low expression of miR-1300 and miR-939 was significantly correlated with shorter distant metastasis-free survival (DMFS) in Cox univariate analysis (p.adjusted = 0.049). The expression signature of five miRNAs (miR-1296, miR-135b, miR-539, miR-572 and miR-185) was found to be prognostic [p = 1.28E−07, HR 8.4 (95 % CI: 3.81–18.52)] for DMFS and cross-validated in a leave-one-out analysis, with the sensitivity and specificity of 74 and 78 %, respectively. The expression of miR-592 was significantly associated with the MMR status (p.adjusted
ISSN:0262-0898
1573-7276
DOI:10.1007/s10585-016-9810-1