Tropisetron Protects Against Acetaminophen-Induced Liver Injury via Suppressing Hepatic Oxidative Stress and Modulating the Activation of JNK/ERK MAPK Pathways

• Published in: BioMed research international 2016-01, Vol.2016 (2016), p.1-9
• Main Authors: Li, Allen H., Liao, Chia-Chih, Lee, Hung-Chen, Liu, Fu-Chao, Yu, Huang-Ping
• Format: Article
• Language: English
• Subjects: Acetaminophen; Acetaminophen - adverse effects; Analgesics; Analysis; Animals; Apoptosis; Aspartate; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - pathology; Chemokines; Cytochrome; Cytokines; Enzymes; Gene expression; Humans; Indoles - administration & dosage; Kinases; Laboratory animals; Liver; Liver - drug effects; Liver - enzymology; Liver - pathology; Liver diseases; Male; MAP Kinase Kinase 4 - biosynthesis; MAP Kinase Kinase 4 - genetics; MAP Kinase Signaling System - drug effects; Metabolism; Metabolites; Mice; Oxidative stress; Oxidative Stress - drug effects; Phosphorylation; Protein kinases; Proteins; Rodents; Studies; Superoxide; Survival analysis
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2016/1952947

Objectives. To investigate the protective effects of tropisetron on acetaminophen- (APAP-) induced liver injury in a mice model. Methods. C57BL/6 male mice were given tropisetron (0.3 to 10 mg/kg) 30 minutes before a hepatotoxic dose of acetaminophen (300 mg/kg) intraperitoneally. Twenty hours after APAP intoxication, sera alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, hepatic myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) activities, and liver histopathological changes were examined. The MAP kinases were also detected by western blotting. Results. Our results showed that tropisetron pretreatment significantly attenuated the acute elevations of the liver enzyme ALT level, hepatic MPO activity, and hepatocytes necrosis in a dose-dependent manner (0.3–10 mg/kg) in APAP-induced hepatotoxicity mice. Tropisetron (1 and 3 mg/kg) suppressed APAP-induced hepatic lipid peroxidation expression and alleviated GSH and SOD depletion. Administration of tropisetron also attenuated the phosphorylation of c-Jun-NH2-terminal protein kinase (JNK) and extracellular signal-regulated kinase (ERK) caused by APAP. Conclusion. Our data demonstrated that tropisetron’s hepatoprotective effect was in part correlated with the antioxidant, which were mediated via JNK and ERK pathways on acetaminophen-induced liver injury in mice.