Characterization of two distinct early post-entry blocks to HIV-1 in common marmoset lymphocytes

• Published in: Scientific reports 2016-11, Vol.6 (1), p.37489-37489, Article 37489
• Main Authors: Pacheco, Beatriz, Menéndez-Arias, Luis, Sodroski, Joseph
• Format: Article
• Language: English
• Subjects: 38/22; 38/39; 38/70; 38/77; 631/326/596/1787; 631/326/596/2556; 631/326/596/2557; Animals; B-Lymphocytes - immunology; B-Lymphocytes - virology; Callithrix - immunology; Callithrix - virology; Capsid Proteins - genetics; Capsid Proteins - immunology; Carrier Proteins - genetics; Carrier Proteins - immunology; Catarrhini; Cell Line; HIV Infections - genetics; HIV Infections - immunology; HIV Infections - virology; HIV-1 - genetics; HIV-1 - immunology; Hominoidea; Humanities and Social Sciences; Humans; Infections; Integration; Lentivirus - genetics; Lentivirus - immunology; Lentivirus - pathogenicity; Lymphocytes; Lymphocytes - immunology; Lymphocytes - virology; Lymphocytes B; Monkeys & apes; multidisciplinary; Peripheral blood; Platyrrhini; Reverse transcription; Science; Virus Internalization; Virus Replication - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep37489

In nature, primate lentiviruses infect humans and several Old World monkeys and apes. However, to date, lentiviruses infecting New World monkeys have not been described. We studied the susceptibility of common marmoset cells to HIV-1 infection and observed the presence of post-entry blocks to the early phase of HIV-1 infection in peripheral blood lymphocytes (PBLs) and a B lymphocytic cell line (B-LCL). The blocks present in these cells are dominant and phenotypically different from each other. In PBLs, the block occurs at the level of reverse transcription, reducing the accumulation of early and late transcripts, similar to the block imposed by TRIM5α. However, we have found that marmoset TRIM5α does not block HIV-1. In contrast, the restriction factor present in B-LCLs blocks HIV-1 replication at a later step, after nuclear entry, and inhibits integration. Additionally, we have identified an HIV-1 capsid mutant, N74D, that is able to escape the restriction in the marmoset B-LCLs. Our results suggest that the factors responsible for the blocks present in marmoset PBLs and B-LCLs are different. We propose the existence of at least two new restriction factors able to block HIV-1 infection in marmoset lymphocytes.