Contributory roles of two l-lactate dehydrogenases for l-lactic acid production in thermotolerant Bacillus coagulans

Thermotolerant Bacillus coagulans is considered to be a more promising producer for bio-chemicals, due to its capacity to withstand harsh conditions. Two L-lactate dehydrogenase (LDH) encoding genes ( ldh L1 and ldh L2) and one D-LDH encoding gene ( ldh D) were annotated from the B. coagulans DSM1 g...

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Bibliographic Details
Published in:Scientific reports 2016-11, Vol.6 (1), p.37916-37916, Article 37916
Main Authors: Sun, Lifan, Zhang, Caili, Lyu, Pengcheng, Wang, Yanping, Wang, Limin, Yu, Bo
Format: Article
Language:English
Subjects:
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Acid production
Acids
Annotations
Bacillus coagulans - enzymology
Bacillus coagulans - genetics
Bacillus coagulans - growth & development
Bacillus coagulans - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Chemicals
Complementation
Dehydrogenases
Engineering
Enzymes
Experiments
Fermentation
Gene deletion
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Genes
Genomes
Humanities and Social Sciences
L-Lactate dehydrogenase
L-Lactate Dehydrogenase - genetics
L-Lactate Dehydrogenase - metabolism
Lactic acid
Lactic Acid - metabolism
Molecular Sequence Annotation
multidisciplinary
NADH
Productivity
Roles
Science
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Summary:Thermotolerant Bacillus coagulans is considered to be a more promising producer for bio-chemicals, due to its capacity to withstand harsh conditions. Two L-lactate dehydrogenase (LDH) encoding genes ( ldh L1 and ldh L2) and one D-LDH encoding gene ( ldh D) were annotated from the B. coagulans DSM1 genome. Transcriptional analysis revealed that the expression of ldh L2 was undetectable while the ldh L1 transcription level was much higher than that of ldh D at all growth phases. Deletion of the ldh L2 gene revealed no difference in fermentation profile compared to the wild-type strain, while ldh L1 single deletion or ldh L1 ldh L2 double deletion completely blocked L-lactic acid production. Complementation of ldh L1 in the above knockout strains restored fermentation profiles to those observed in the wild-type strain. This study demonstrates ldh L1 is crucial for L-lactic acid production and NADH balance in B. coagulans DSM1 and lays the fundamental for engineering the thermotolerant B. coagulans strain as a platform chemicals producer.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep37916