Suppression of OsVPE3 Enhances Salt Tolerance by Attenuating Vacuole Rupture during Programmed Cell Death and Affects Stomata Development in Rice

Background Vacuolar processing enzymes (VPEs) are cysteine proteinases that act as crucial mediators of programmed cell death (PCD) in plants. In rice, however, the role of VPEs in abiotic stress-induced PCD remains largely unknown. In this study, we generated OsVPE3 overexpression and suppression t...

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Published in:Rice (New York, N.Y.) N.Y.), 2016-12, Vol.9 (1), p.65-65, Article 65
Main Authors: Lu, Wenyun, Deng, Minjuan, Guo, Fu, Wang, Mingqiang, Zeng, Zhanghui, Han, Ning, Yang, Yinong, Zhu, Muyuan, Bian, Hongwu
Format: Article
Language:English
Subjects:
Agriculture
Apoptosis
Biomedical and Life Sciences
chlorophyll
cysteine proteinases
DNA
Enzymes
gene expression regulation
gene overexpression
genes
guard cells
Life Sciences
Original
Original Article
Oryza sativa
Plant Breeding/Biotechnology
Plant Ecology
Plant Genetics and Genomics
Plant Sciences
plasma membrane
Rice
Salt
salt stress
salt tolerance
staining
stomata
stress tolerance
survival rate
transgenic plants
vacuoles
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Summary:Background Vacuolar processing enzymes (VPEs) are cysteine proteinases that act as crucial mediators of programmed cell death (PCD) in plants. In rice, however, the role of VPEs in abiotic stress-induced PCD remains largely unknown. In this study, we generated OsVPE3 overexpression and suppression transgenic lines to elucidate the function of this gene in rice. Results Survival rate and chlorophyll retention analyses showed that suppression of OsVPE3 clearly enhanced salt stress tolerance in transgenic rice compared with wild type. Furthermore, fragmentation of genomic DNA was inhibited in plants with down-regulated OsVPE3 . Vital staining studies indicated that vacuole rupture occurred prior to plasma membrane collapse during salt-induced PCD. Notably, overexpression of OsVPE3 promoted vacuole rupture, whereas suppression of OsVPE3 attenuated or delayed the disintegration of vacuolar membranes. Moreover, we found that suppression of OsVPE3 caused decreased leaf width and guard cell length in rice. Conclusions Taken together, these results indicated that suppression of OsVPE3 enhances salt tolerance by attenuating vacuole rupture during PCD. Therefore, we concluded that OsVPE3 plays a crucial role in vacuole-mediated PCD and in stomatal development in rice.
ISSN:1939-8425
1939-8433
1934-8037
DOI:10.1186/s12284-016-0138-x