Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro

Ayurved Siriraj Wattana recipe (AVS073), has been prescribed as tonic, to increase appetite, and for pain relief. It also exhibits antioxidant, anti-inflammatory, immunomodulating and anti-cancer activities. However, the immunomodulatory effects on antigen-presenting cells and effector T cells remai...

Full description

Saved in:
Bibliographic Details
Published in:BMC complementary and alternative medicine 2016-11, Vol.16 (1), p.489-489, Article 489
Main Authors: Wongkajornsilp, Adisak, Numchaisermsuk, Nuntarak, Sa-Ngiamsuntorn, Khanit, Akarasereenont, Pravit, Wamanuttajinda, Valla, Kasetsinsombat, Kanda, Duangsa-Ard, Sunisa, Laohapan, Tawee, Maneechotesuwan, Kittipong
Format: Article
Language:English
Subjects:
Antioxidants
CD3 Complex
CD56 Antigen
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells, Cultured
Cytokine-Induced Killer Cells - drug effects
Cytokines - metabolism
Dendritic cells
Dendritic Cells - drug effects
Folk medicine
Humans
Immunity
Immunophenotyping
Medicine, Primitive
Physiological aspects
Plant Preparations - pharmacology
Plants, Medicinal - chemistry
T-Lymphocyte Subsets - drug effects
Thailand
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ayurved Siriraj Wattana recipe (AVS073), has been prescribed as tonic, to increase appetite, and for pain relief. It also exhibits antioxidant, anti-inflammatory, immunomodulating and anti-cancer activities. However, the immunomodulatory effects on antigen-presenting cells and effector T cells remained elusive. We thus aimed to study the effects of AVS073 on differentiation, maturation, functions and proportions of CIK cells and monocyte-derived DCs. CIK cells and monocyte-derived DCs were treated with AVS073, followed by the assessment of T-helper (Th) phenotypes using real-time RT-PCR and flow cytometry. AVS073 promoted Th1 phenotype in CD3 CD56 subset of CIK cells through increasing STAT4, T-bet, and interferon-γ. AVS073 inhibited Th2 phenotype through decreasing STAT6. AVS073 inhibited Treg phenotype through decreasing STAT5A, STAT5B and IDO. AVS073 promoted Th17 phenotype through increasing STAT3, RORC and IL-17. AVS073 treatment of mDCs resulted in increasing Th1-prone cytokine (IL-12) and Th17-prone cytokines (IL-6 and IL-23). AVS073 upregulated Th1 and Th17, but downregulated Th2 and Treg phenotypes within CD3 CD56 cells. The treatment of mDCs drove Th1 and Th17-polarizations.
ISSN:1472-6882
1472-6882
DOI:10.1186/s12906-016-1480-7