XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE

• Published in: Cell death & disease 2016-10, Vol.7 (10), p.e2409-e2409
• Main Authors: Hou, Shuai, Li, Na, Zhang, Qian, Li, Hui, Wei, Xinyue, Hao, Tian, Li, Yue, Azam, Sikandar, Liu, Caigang, Cheng, Wei, Jin, Bilian, Liu, Quentin, Li, Man, Lei, Haixin
• Format: Article
• Language: English
• Subjects: 13/109; 13/89; 14/63; 38/61; 631/337/572; 631/67/1059; 631/80/641/1655; 631/80/86; 82/1; Antibodies; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Culture; Cell cycle; Cell Cycle Checkpoints - genetics; Cell Death - genetics; Cell division; Cell Nucleus - metabolism; Chromosomal Proteins, Non-Histone - genetics; Chromosomal Proteins, Non-Histone - metabolism; Chromosome Segregation - genetics; DNA Breaks; Gene Deletion; Gene expression; Gene Expression Regulation; Gene Knockdown Techniques; HEK293 Cells; HeLa Cells; Humans; Immunology; Life Sciences; Microtubules - metabolism; Mitosis - genetics; Oligonucleotide Array Sequence Analysis; Original; original-article; Spindle Apparatus - metabolism; Transcription Factors - deficiency; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/cddis.2016.313

Xeroderma pigmentosum group A (XPA)-binding protein 2 (XAB2) is a multi-functional protein that plays critical role in processes including transcription, transcription-coupled DNA repair, pre-mRNA splicing, homologous recombination and mRNA export. Microarray analysis on gene expression in XAB2 knockdown cells reveals that many genes with significant change in expression function in mitotic cell cycle regulation. Fluorescence-activated cell scanner analysis confirmed XAB2 depletion led to cell arrest in G2/M phase, mostly at prophase or prometaphase. Live cell imaging further disclosed that XAB2 knockdown induced severe mitotic defects including chromosome misalignment and defects in segregation, leading to mitotic arrest, mitotic catastrophe and subsequent cell death. Among top genes down-regulated by XAB2 depletion is mitotic motor protein centrosome-associated protein E (CENPE). Knockdown CENPE showed similar phenotypes to loss of XAB2, but CENPE knockdown followed by XAB2 depletion did not further enhance cell cycle arrest. Luciferase assay on CENPE promoter showed that overexpression of XAB2 increased luciferase activity, whereas XAB2 depletion resulted in striking reduction of luciferase activity. Further mapping revealed a region in CENPE promoter that is required for the transcriptional regulation by XAB2. Moreover, ChIP assay showed that XAB2 interacted with CENPE promoter. Together, these results support a novel function of XAB2 in mitotic cell cycle regulation, which is partially mediated by transcription regulation on CENPE.