Contrasting biological potency of particulate matter collected at sites impacted by distinct industrial sources

Industrial sources contribute a significant proportion of anthropogenic particulate matter (PM) emissions, producing particles of varying composition that may differentially impact health. This study investigated the in vitro toxicity of ambient PM collected near industrial sites in relation to part...

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Bibliographic Details
Published in:Particle and fibre toxicology 2016-12, Vol.13 (1), p.65-65, Article 65
Main Authors: Thomson, Errol M, Breznan, Dalibor, Karthikeyan, Subramanian, MacKinnon-Roy, Christine, Vuong, Ngoc Q, Dabek-Zlotorzynska, Ewa, Celo, Valbona, Charland, Jean-Pierre, Kumarathasan, Prem, Brook, Jeffrey R, Vincent, Renaud
Format: Article
Language:English
Subjects:
Air pollution
Airborne particulates
Animals
Cell Line
Copper
Cytokines - metabolism
Cytotoxicity
Health aspects
Heart rate
Humans
Industry
Mice
Mortality
Outdoor air quality
Particles
Particulate Matter - toxicity
Polycyclic aromatic hydrocarbons
Toxicity
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Summary:Industrial sources contribute a significant proportion of anthropogenic particulate matter (PM) emissions, producing particles of varying composition that may differentially impact health. This study investigated the in vitro toxicity of ambient PM collected near industrial sites in relation to particle size and composition. Size-fractionated particles (ultrafine, PM , PM , PM ) were collected in the vicinity of steel, copper, aluminium, and petrochemical industrial sites. Human lung epithelial-like A549 and murine macrophage-like J774A.1 cells were exposed for 24 h to particle suspensions (0, 30, 100, 300 μg/cm ). Particle potency was assessed using cytotoxic (resazurin reduction, lactate dehydrogenase (LDH) release) and inflammatory (cytokine release) assays, and regressed against composition (metals, polycyclic aromatic hydrocarbons (PAHs), endotoxin). Coarse (PM , PM ) particle fractions were composed primarily of iron and aluminium; in contrast, ultrafine and fine (PM ) fractions displayed considerable variability in metal composition (especially water-soluble metals) across collection sites consistent with source contributions. Semi-volatile and PM-associated PAHs were enriched in the fine and coarse fractions collected near metal industry. Cell responses to exposure at equivalent mass concentrations displayed striking differences among sites (SITE x SIZE and SITE x DOSE interactions, p 
ISSN:1743-8977
1743-8977
DOI:10.1186/s12989-016-0176-y