The transcriptomic G1–G6 signature of hepatocellular carcinoma in an Asian population: Association of G3 with microvascular invasion

In this study, a transcriptomic group classification based on a European population is tested on a Singapore cohort. The results highlight the genotype/phenotype correlation in a Southeast Asian population. The G1-G6 transcriptomic classification derived from hepatocellular carcinoma (HCC) resected...

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Bibliographic Details
Published in:Medicine (Baltimore) 2016-11, Vol.95 (47), p.e5263-e5263
Main Authors: Allen, John Carson, Nault, Jean-Charles, Zhu, Guili, Khor, Andrew Yu Keat, Liu, Jin, Lim, Tony Kiat Hon, Zucman-Rossi, Jessica, Chow, Pierce K.H.
Format: Article
Language:English
Subjects:
Aged
Carcinoma, Hepatocellular - classification
Carcinoma, Hepatocellular - ethnology
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - surgery
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genes, Neoplasm
Genotype
Humans
Liver Neoplasms - classification
Liver Neoplasms - ethnology
Liver Neoplasms - genetics
Liver Neoplasms - surgery
Male
Middle Aged
Neoplasm Invasiveness - genetics
Observational Study
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Singapore
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Summary:In this study, a transcriptomic group classification based on a European population is tested on a Singapore cohort. The results highlight the genotype/phenotype correlation in a Southeast Asian population. The G1-G6 transcriptomic classification derived from hepatocellular carcinoma (HCC) resected from European patients, robustly reflected group-specific clinical/pathological features. We investigated the application of this molecular classification in Southeast Asian HCC patients.Gene expression analysis was carried out on HCC surgically resected in Singapore patients who were grouped into G1-G6 transcriptomic categories according to expression of 16 predictor genes (illustrated in Supplementary Table 1, http://links.lww.com/MD/B413 and Supplementary Fig. 1, http://links.lww.com/MD/B413) using quantitative reverse transcription polymerase chain reaction (RT-PCR). Univariate and multivariate polytomous logistic regression was used to investigate association between clinical variables and pooled transcriptomic classes G12, G3, and G456.HCC from Singapore (n = 82) were distributed (%) into G1 (13.4), G2 (24.4), G3 (15.9), G4 (24.4), G5 (14.6), and G6 (7.3) subgroups. Compared to the European data, the Singapore samples were relatively enriched in G1-G3 versus G4-G6 tumors (53.7% vs 46.3%) reflecting the higher proportion of hepatitis B virus (HBV) patients in Singapore versus Europe samples (43% vs 30%). Pooled classes were defined as G12, G3, and G456. G12 was associated with higher alpha-fetoprotein (AFP) concentrations (OR = 1.69, 95% CI: 1.30-2.20; P 
ISSN:0025-7974
1536-5964
DOI:10.1097/MD.0000000000005263