CTGF enhances resistance to 5-FU-mediating cell apoptosis through FAK/MEK/ERK signal pathway in colorectal cancer

Colorectal cancer (CRC) is one of the most commonly diagnosed cancers among both males and females; the chemotherapy drug 5-fluorouracil (5-FU) is one of a doctors' first lines of defense against CRC. However, therapeutic failures are common because of the emergence of drug resistance. Connecti...

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Bibliographic Details
Published in:OncoTargets and therapy 2016-01, Vol.9, p.7285-7295
Main Authors: Yang, Kai, Gao, Kai, Hu, Gui, Wen, Yanguang, Lin, Changwei, Li, Xiaorong
Format: Article
Language:English
Subjects:
5-Fluorouracil
Apoptosis
Biotechnology
Bone cancer
Cancer therapies
Care and treatment
Cell cycle
Cellular signal transduction
Chemotherapy
Colorectal cancer
Development and progression
Diagnosis
Drug resistance
Gene expression
Genetic aspects
Growth factors
Health aspects
Kinases
Lymphoma
Metastasis
Original Research
Phosphatidylinositols
Proteins
Tumors
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Summary:Colorectal cancer (CRC) is one of the most commonly diagnosed cancers among both males and females; the chemotherapy drug 5-fluorouracil (5-FU) is one of a doctors' first lines of defense against CRC. However, therapeutic failures are common because of the emergence of drug resistance. Connective tissue growth factor (CTGF) is a secreted protein that binds to integrins, and regulates the invasiveness and metastasis of certain carcinoma cells. Here, we found that CTGF was upregulated in drug-resistant phenotype of human CRC cells. Overexpression of CTGF enhanced the resistance to 5-FU-induced cell apoptosis. Moreover, downregulating the expression of CTGF promoted the curative effect of chemotherapy and blocked the cell cycle in the G1 phase. We also found that CTGF facilitated resistance to 5-FU-induced apoptosis by increasing the expression of B-cell lymphoma-extra large (Bcl-xL) and survivin. Then we pharmacologically blocked MEK/ERK signal pathway and assessed 5-FU response by MTT assays. Our current results indicate that the expression of phosphorylated forms of MEK/ERK increased in high CTGF expression cells and MEK inhibited increases in 5-FU-mediated apoptosis of resistant CRC cells. Therefore, our data suggest that MEK/ERK signaling contributes to 5-FU resistance through upstream of CTGF, and supports CRC cell growth. Comprehending the molecular mechanism underlying 5-FU resistance may ultimately aid the fight against CRC.
ISSN:1178-6930
1178-6930
DOI:10.2147/OTT.S108929