L-Asparaginase Activity in Cell Lysates and Culture Media of Halophilic Bacterial Isolates

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among...

Full description

Saved in:
Bibliographic Details
Published in:Iranian journal of pharmaceutical research : IJPR 2016-01, Vol.15 (3), p.435-440
Main Authors: Barati, Mahmood, Faramarzi, Mohammad Ali, Nafissi-Varcheh, Nastaran, Khoshayand, Mohammad Reza, Houshdar Tehrani, Mohammad Hassan, Vahidi, Hossein, Adrangi, Sina
Format: Article
Language:English
Subjects:
Original
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to grow in the selection medium containing 3.5 M NaCl. None of the isolates appeared to produce appreciable amounts of extracellular L-asparaginase. Among the isolates that produced intracellular L-asparaginase, 5 moderate and 1 extreme halophiles were selected for further study based on their observed activity level. The moderately halophilic isolates were shown to belong to the genus while the extreme halophile was identified as a member of the genus .
ISSN:1735-0328
1726-6890