Background Suppression in Imaging Gold Nanorods through Detection of Anti-Stokes Emission

Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image go...

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Published in:Biophysical journal 2016-12, Vol.111 (11), p.2492-2499
Main Authors: Carattino, Aquiles, Keizer, Veer I.P., Schaaf, Marcel J.M., Orrit, Michel
Format: Article
Language:English
Subjects:
Emissions
Fluorescence
Gold
Gold - chemistry
Gold - metabolism
HeLa Cells
Humans
Image Processing, Computer-Assisted
Luminescence
Membranes
Microscopy, Fluorescence
Molecular Machines, Motors, and Nanoscale Biophysics
Nanoparticles
Nanotubes - chemistry
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cited_by cdi_FETCH-LOGICAL-c479t-b7339829aeb22b96ecf42273b7b6172425f5009c5b15219ccbc4e8778e958d0a3
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container_issue 11
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container_title Biophysical journal
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creator Carattino, Aquiles
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description Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by detecting their anti-Stokes emission under resonant excitation. We show that even in the membrane of a cell containing the fluorescent dye Atto 647N, the signal/background of the anti-Stokes emission can be >10, while it is impossible to image the particles with the Stokes emission. The main advantage of this technique is that it does not require any major change in existing fluorescence imaging setups, only the addition of an appropriate short-pass filter in the detection path.
doi_str_mv 10.1016/j.bpj.2016.10.035
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subjects Emissions
Fluorescence
Gold
Gold - chemistry
Gold - metabolism
HeLa Cells
Humans
Image Processing, Computer-Assisted
Luminescence
Membranes
Microscopy, Fluorescence
Molecular Machines, Motors, and Nanoscale Biophysics
Nanoparticles
Nanotubes - chemistry
title Background Suppression in Imaging Gold Nanorods through Detection of Anti-Stokes Emission
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