Effects of fluctuating temperature and food availability on reproduction and lifespan

• Published in: Experimental gerontology 2016-12, Vol.86, p.62-72
• Main Authors: Schwartz, Tonia S., Pearson, Phillip, Dawson, John, Allison, David B., Gohlke, Julia M.
• Format: Article
• Language: English
• Subjects: Adaptive plasticity; Aging - physiology; Animals; Caloric Restriction; Daphnia - physiology; Dietary restriction; Energy Metabolism - physiology; Fecundity; Fertility - physiology; Food; Intergenerational effects; Life span; Longevity - physiology; Reproduction - physiology; Survival Rate; Temperature; Thermal fluctuations
• ISSN: 0531-5565; 1873-6815
• DOI: 10.1016/j.exger.2016.06.010

Experimental studies on energetics and aging often remove two major factors that in part regulate the energy budget in a normal healthy individual: reproduction and fluctuating environmental conditions that challenge homeostasis. Here we use the cyclical parthenogenetic Daphnia pulex to evaluate the role of a fluctuating thermal environment on both reproduction and lifespan across six food concentrations. We test the hypotheses that (1) caloric restriction extends lifespan; (2) maximal reproduction will come with a cost of shortened lifespan; and (3) at a given food concentration, relative to a metabolically equivalent constant temperature environment a diel fluctuating thermal environment will alter the allocation of energy to reproduction and lifespan to maintain homeostasis. We did not identify a level of food concentration that extended lifespan in response to caloric restriction, and we found no cost of reproduction in terms of lifespan. Rather, the individuals at the highest food levels generally had the highest reproductive output and the longest lifespans, the individuals at the intermediate food level decreased reproduction and maintained lifespan, and the individuals at the three lower food concentrations had a decrease in reproduction and lifespan as would be predicted with increasing levels of starvation. Fluctuating temperature had no effect on lifespan at any food concentration, but delayed time to reproductive maturity and decreased early reproductive output at all food concentrations. This suggests that a fluctuating temperature regimen activates molecular pathways that alter energy allocation. The costs of fluctuating temperature on reproduction were not consistent across the lifespan. Statistical interactions for age of peak reproduction and lifetime fecundity suggest that senescence of the reproductive system may vary between temperature regimens at the different food concentrations. •A daily fluctuating temperature regimen delays reproduction in Daphnia compared to an equivalent constant temperature.•Additional energy input does not eliminate the cost of fluctuating temperature on early life reproduction in daphnia.•Fluctuating temperature had minimal effect on lifespan.•Caloric restriction at the chosen food levels did not extend lifespan.•No cost of reproduction was realized in terms of lifespan at maximal energy input.