Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing

Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regu...

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Bibliographic Details
Published in:The Journal of biological chemistry 2016-12, Vol.291 (51), p.26377-26387
Main Authors: Wang, Ziqiang, Liu, Qing, Lu, Jinhua, Fan, Ping, Xie, Weidong, Qiu, Wei, Wang, Fan, Hu, Guangnan, Zhang, Yaou
Format: Article
Language:English
Subjects:
Animals
Chlorocebus aethiops
gene expression
Gene Expression Regulation, Viral - physiology
HeLa Cells
herpesvirus
Herpesvirus 1, Human - physiology
host-pathogen interaction
Humans
Immediate-Early Proteins - biosynthesis
Immediate-Early Proteins - genetics
Microbiology
RNA Precursors - biosynthesis
RNA Precursors - genetics
RNA splicing
RNA Splicing - physiology
RNA, Viral - biosynthesis
RNA, Viral - genetics
Serine-Arginine Splicing Factors - genetics
Serine-Arginine Splicing Factors - metabolism
transcription factor
Transcription, Genetic - physiology
Ubiquitin-Protein Ligases - biosynthesis
Ubiquitin-Protein Ligases - genetics
Vero Cells
Virus Replication - physiology
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Summary:Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regulating viral gene expression at the transcriptional and posttranscriptional levels. Our results indicate that SRSF2 functions as a transcriptional activator by directly binding to infected cell polypeptide 0 (ICP0), infected cell polypeptide 27 (ICP27), and thymidine kinase promoters. Moreover, SRSF2 participates in ICP0 pre-mRNA splicing by recognizing binding sites in ICP0 exon 3. These findings provide insight into the functions of SRSF2 in HSV-1 replication and gene expression.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M116.753046