LncRNA HULC enhances epithelial-mesenchymal transition to promote tumorigenesis and metastasis of hepatocellular carcinoma via the miR-200a-3p/ZEB1 signaling pathway

• Published in: Oncotarget 2016-07, Vol.7 (27), p.42431-42446
• Main Authors: Li, Shi-Peng, Xu, Hai-Xu, Yu, Yao, He, Jin-Dan, Wang, Zhen, Xu, Yan-Jie, Wang, Chang-Ying, Zhang, Hai-Ming, Zhang, Rong-Xin, Zhang, Jian-Jun, Yao, Zhi, Shen, Zhong-Yang
• Format: Article
• Language: English
• Subjects: Acetylcysteine - metabolism; Aged; Animals; Apoptosis; Carcinogenesis; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Cell Proliferation; Cell Transformation, Neoplastic - genetics; Disease Progression; Epithelial-Mesenchymal Transition; Female; Humans; Lentivirus - genetics; Liver - metabolism; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Male; Mice; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Neoplasm Metastasis; Neoplasm Transplantation; Research Paper; RNA, Long Noncoding - metabolism; RNA, Small Interfering - metabolism; Signal Transduction; Zinc Finger E-box-Binding Homeobox 1 - genetics; Zinc Finger E-box-Binding Homeobox 1 - metabolism
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.9883

Highly upregulated in liver cancer (HULC), a lncRNA that is considered a key molecule in human liver cancer, has recently been revealed to be involved in hepatocellular carcinoma (HCC) development and progression [1, 2]. It has been reported that HULC can promote tumor invasion and metastasis of HCC, but its function and mechanism of action in HCC have not been elucidated. In this study, we found that HULC was aberrantly up-regulated in HCC tissues and associated with TNM stage, intrahepatic metastases, HCC recurrence, and postoperative survival. HULC depletion inhibited the growth and metastasis of HCC cell lines in vitro and in vivo. Moreover, HULC contributes to ZEB1-induced epithelial-mesenchymal transition (EMT), a requirement for tumor invasion and metastasis that plays a key role in cancer progression. This effect of ZEB1 was inhibited by HULC siRNA. We conclude that the HULC functioned as a competing endogenous RNA (ceRNA) to mediate EMT via up-regulating ZEB1. In this way, it sequesters the miR-200a-3p signaling pathway to facilitate HCC metastasis. HULC comes into play as an oncogene in HCC, acting mechanistically by inducing HCC cells to activate EMT. Such an effect promotes tumor progression and metastasis through the miR-200a-3p/ZEB1 signaling pathway. The identification of this novel pathway that links high expression levels of HULC with EMT in HCC cells may serve as the foundation for the development of novel anti-tumor therapeutics.