Sugar-Modified Poly(propylene imine) Dendrimers Stimulate the NF-κB Pathway in a Myeloid Cell Line

• Published in: Pharmaceutical research 2017-01, Vol.34 (1), p.136-147
• Main Authors: Jatczak-Pawlik, Izabela, Gorzkiewicz, Michal, Studzian, Maciej, Appelhans, Dietmar, Voit, Brigitte, Pulaski, Lukasz, Klajnert-Maculewicz, Barbara
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Cell Line; Dendrimers - administration & dosage; Dendrimers - chemistry; Humans; Immunity, Innate - drug effects; Immunity, Innate - immunology; Interleukin-4 - immunology; Maltose - chemistry; Maltose - immunology; Medical Law; Monocytes - drug effects; Monocytes - immunology; Myeloid Cells - drug effects; Myeloid Cells - immunology; NF-kappa B - immunology; Pharmacology/Toxicology; Pharmacy; Polypropylenes - administration & dosage; Polypropylenes - chemistry; Polypropylenes - immunology; Research Paper; RNA, Messenger - immunology; Signal Transduction - drug effects; Signal Transduction - immunology; Sugars - administration & dosage; Sugars - chemistry; Sugars - immunology; Transcription Factor RelA - immunology
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-016-2049-3

Purpose Fourth-generation poly(propylene imine) dendrimers fully surface-modified by maltose (dense shell, PPI-m DS) were shown to be biocompatible in cellular models, which is important for their application in drug delivery. We decided to verify also their inherent bioactivity, including immunomodulatory activity, for potential clinical applications. We tested their effects on the THP-1 monocytic cell line model of innate immunity effectors. Methods To estimate the cytotoxicity of dendrimers the reasazurin assay was performed. The expression level of NF-κB targets: IGFBP3 , TNFAIP3 and TNF was determined by quantitative real-time RT-PCR. Measurement of NF-κB p65 translocation from cytoplasm to nucleus was conducted with a high-content screening platform and binding of NF-κB to a consensus DNA probe was determined by electrophoretic mobility shift assay. The cytokine assay was performed to measure protein concentration of TNFalpha and IL-4. Results We found that PPI-m DS did not impact THP-1 viability and growth even at high concentrations (up to 100 μM). They also did not induce expression of genes for important signaling pathways: Jak/STAT, Keap1/Nrf2 and ER stress. However, high concentrations of 4th generation PPI-m DS (25–100 μM), but not their 3rd generation counterparts, induced nuclear translocation of p65 NF-κB protein and its DNA-binding activity, leading to NF-κB-dependent increased expression of mRNA for NF-κB targets: IGFBP3 , TNFAIP3 and TNF . However, no increase in pro-inflammatory cytokine secretion was detected. Conclusion We conclude that maltose-modified PPI dendrimers of specific size could exert a modest immunomodulatory effect, which may be advantageous in clinical applications (e.g. adjuvant effect in anti-cancer vaccines).