Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion

Twenty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition,...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-14
Main Authors: Hun, Tan Geok, Al-Obaidi, J. R., Neela, Vasantha Kumari, Abdul Rahim, Raha, Dakheel, Khulood Hamid, Yusoff, Khatijah
Format: Article
Language:English
Subjects:
Aggregates
Bacteria
Bacterial Adhesion - drug effects
Bacterial Adhesion - genetics
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biomedical research
Biotechnology
Cell adhesion
Deoxyribonucleic acid
DNA
Enzymes
Genes
Humans
Methicillin Resistance - drug effects
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - growth & development
Microbial mats
Nosocomial infections
Observations
Periodic Acid - pharmacology
Physiological aspects
Proteins
Staphylococcal Infections - drug therapy
Staphylococcal Infections - genetics
Staphylococcal Infections - microbiology
Staphylococcal Protein A - genetics
Staphylococcal Protein A - metabolism
Staphylococcus aureus
Staphylococcus infections
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Summary:Twenty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition, cell adhesion to surfaces and cell cohesion were evaluated using the packed-bead method and mechanical disruption, respectively. The predominant genotype was spa type t127 (22 out of 25 isolates); the majority of isolates were categorized as moderate biofilm producers. Twelve isolates displayed PIA-independent biofilm formation, while the remaining 13 isolates were PIA-dependent. Both groups showed strong dispersal in response to RNase and DNase digestion followed by proteinase K treatment. PIA-dependent biofilms showed variable dispersal after sodium metaperiodate treatment, whereas PIA-independent biofilms showed enhanced biofilm formation. There was no correlation between the extent of biofilm formation or biofilm components and the adhesion or cohesion abilities of the bacteria, but the efficiency of adherence to glass beads increased after biofilm depletion. In conclusion, nucleic acids and proteins formed the main components of the MRSA clone t127 biofilm matrix, and there seems to be an association between adhesion and cohesion in the biofilms tested.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/4708425