Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations

Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used fo...

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Published in:BMC genomics 2017-01, Vol.18 (1), p.14-14, Article 14
Main Authors: Capra, E, Turri, F, Lazzari, B, Cremonesi, P, Gliozzi, T M, Fojadelli, I, Stella, A, Pizzi, F
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Abnormalities
AKT protein
Animals
Apoptosis
Breeding
Cattle
Cell Separation - methods
Cluster Analysis
Cryopreservation
Fertility
Flow cytometry
Gene Expression Regulation
Gene sequencing
Genomes
Genomics
Health aspects
High-Throughput Nucleotide Sequencing
Identification and classification
Infertility
Male
MicroRNA
MicroRNAs
MicroRNAs - genetics
miRNA
Models, Biological
Motility
Populations
PTEN protein
Ribonucleic acid
RNA
RNA Interference
RNA sequencing
RNA, Small Interfering - genetics
RNA, Small Untranslated - genetics
Semen
Semen - metabolism
Signal Transduction
Software
Sperm
Sperm Motility - genetics
Spermatogenesis
Spermatozoa
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cited_by cdi_FETCH-LOGICAL-c556t-b141c84ffc35662ffa25f1125b3175063531df8e4e443829cc6ad7e8fcac3dc23
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container_title BMC genomics
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Fojadelli, I
Stella, A
Pizzi, F
description Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used for breeding, a method based on deep sequencing of sperm microRNA (miRNA) and Piwi-interacting RNA (piRNA) from individual bulls was developed. To validate our method, two populations of spermatozoa isolated from high and low motile fractions separated by Percoll were sequenced, and their small RNAs content characterized. Sperm cells from frozen thawed semen samples of 4 bulls were successfully separated in two fractions. We identified 83 miRNAs and 79 putative piRNAs clusters that were differentially expressed in both fractions. Gene pathways targeted by 40 known differentially expressed miRNAs were related to apoptosis. Dysregulation of miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184 and miR-20a-5p was found to target three pathways (PTEN, PI3K/AKT and STAT). Small RNAs sequencing data obtained from single bulls are consistent with previous findings. Specific miRNAs are differentially represented in low versus high motile sperm, suggesting an alteration of cell functions and increased germ cell apoptosis in the low motile fraction.
doi_str_mv 10.1186/s12864-016-3394-7
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At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used for breeding, a method based on deep sequencing of sperm microRNA (miRNA) and Piwi-interacting RNA (piRNA) from individual bulls was developed. To validate our method, two populations of spermatozoa isolated from high and low motile fractions separated by Percoll were sequenced, and their small RNAs content characterized. Sperm cells from frozen thawed semen samples of 4 bulls were successfully separated in two fractions. We identified 83 miRNAs and 79 putative piRNAs clusters that were differentially expressed in both fractions. Gene pathways targeted by 40 known differentially expressed miRNAs were related to apoptosis. Dysregulation of miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184 and miR-20a-5p was found to target three pathways (PTEN, PI3K/AKT and STAT). 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subjects 1-Phosphatidylinositol 3-kinase
Abnormalities
AKT protein
Animals
Apoptosis
Breeding
Cattle
Cell Separation - methods
Cluster Analysis
Cryopreservation
Fertility
Flow cytometry
Gene Expression Regulation
Gene sequencing
Genomes
Genomics
Health aspects
High-Throughput Nucleotide Sequencing
Identification and classification
Infertility
Male
MicroRNA
MicroRNAs
MicroRNAs - genetics
miRNA
Models, Biological
Motility
Populations
PTEN protein
Ribonucleic acid
RNA
RNA Interference
RNA sequencing
RNA, Small Interfering - genetics
RNA, Small Untranslated - genetics
Semen
Semen - metabolism
Signal Transduction
Software
Sperm
Sperm Motility - genetics
Spermatogenesis
Spermatozoa
title Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
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