Assessment of apoptosis in the native vein used for hemodialysis access

To determine whether apoptosis is more common in previously punctured native veins than in non-punctured native veins among patients who undergo surgical creation of arteriovenous fistula (AVF) for dialysis access. Cephalic vein specimens were obtained from January 1, 2013 to December 31, 2014 from...

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Bibliographic Details
Published in:Croatian medical journal 2016-12, Vol.57 (6), p.540-547
Main Authors: Leci-Tahiri, Laura, Krušlin, Božo, Vučić, Majda, Sonicki, Zdenko, Lovričević, Ivo
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - immunology
Apoptosis - physiology
Arteriovenous Shunt, Surgical
Caspase 3 - biosynthesis
Clinical Science
Health aspects
Hemodialysis patients
Humans
Physiological aspects
Punctures
Renal Dialysis
Time Factors
Veins - physiopathology
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Summary:To determine whether apoptosis is more common in previously punctured native veins than in non-punctured native veins among patients who undergo surgical creation of arteriovenous fistula (AVF) for dialysis access. Cephalic vein specimens were obtained from January 1, 2013 to December 31, 2014 from 60 patients, 30 with previously punctured native veins and 30 with non-punctured native veins. Before AVF placement, a 1-cm vein segment was excised from distal part of the vein for histological, histochemical, and immunohistochemical analysis. Vein specimens were divided into two portions along the longitudinal axis and stained with hematoxylin and eosin for routine histological evaluation. Immunohistochemical analysis was used to localize Bax, p53, caspase 3, and Bcl-2 expression. The group with previously punctured veins showed significantly increased caspase 3 (P
ISSN:0353-9504
1332-8166
DOI:10.3325/cmj.2016.57.540