FNDC3B promotes cell migration and tumor metastasis in hepatocellular carcinoma

Recurrence and metastasis are common in hepatocellular carcinoma (HCC) and correlate with poor prognosis. We investigated the role of fibronectin type III domain containing 3B (FNDC3B) in HCC metastasis. Overexpression of FNDC3B in HCC cell lines enhanced cell migration and invasion. On the other ha...

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Bibliographic Details
Published in:Oncotarget 2016-08, Vol.7 (31), p.49498-49508
Main Authors: Lin, Chin-Hui, Lin, Yao-Wen, Chen, Ying-Chun, Liao, Chen-Chung, Jou, Yuh-Shan, Hsu, Ming-Ta, Chen, Chian-Feng
Format: Article
Language:English
Subjects:
Animals
Annexin A2 - metabolism
Biomarkers, Tumor - metabolism
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - mortality
Cell Line, Tumor
Cell Movement
Chromatography, Liquid
Fibronectins - metabolism
Humans
Kaplan-Meier Estimate
Liver Neoplasms - metabolism
Liver Neoplasms - mortality
Mice
Mice, Inbred BALB C
Mutagenesis
Neoplasm Invasiveness
Neoplasm Metastasis
Oligonucleotide Array Sequence Analysis
Research Paper
RNA, Small Interfering - metabolism
Tandem Mass Spectrometry
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Summary:Recurrence and metastasis are common in hepatocellular carcinoma (HCC) and correlate with poor prognosis. We investigated the role of fibronectin type III domain containing 3B (FNDC3B) in HCC metastasis. Overexpression of FNDC3B in HCC cell lines enhanced cell migration and invasion. On the other hand, knockdown of FNDC3B using short-hairpin RNA reduced tumor nodule formation in both intra- and extra-hepatic metastasis. High levels of FNDC3B were observed in metastatic HCCs and correlated with poor patient survival and shorter recurrence time. Mutagenesis and LC-MS/MS analyses showed that FNDC3B promotes cell migration by cooperating with annexin A2 (ANXA2). Furthermore, FNDC3B and ANXA2 expression correlated negatively with patient survival. Our results indicate that FNDC3B behaves like an oncogene by promoting cell migration. This suggests FNDC3B could serve as a biomarker and therapeutic target for HCC metastasis.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.10374