Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon

Background. Cameroon this last decade continues to present a low contribution of M. africanum and M. bovis in human tuberculosis (TB), while M. bovis was prevalent in cattle but all these pieces of information only concerned West and Center regions. Methods. We carried out the first study in Adamaou...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-12
Main Authors: Eyangoh, Sara, Kuaban, Christopher, Gutierrez, Cristina, Noeske, Juergen, Kaiyven, Afi Leslie, Um Boock, Alphonse, Koro Koro, Francioli, Etoa, François-Xavier
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Animals
Antitubercular Agents - therapeutic use
Cameroon
Cattle
Causes of
Cross-Sectional Studies
Drug resistance
Drug Resistance, Microbial - genetics
Female
Genetic aspects
Health aspects
Humans
Male
Methods
Microbial sensitivity tests
Middle Aged
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - growth & development
Public health
Pulmonary tuberculosis
Sputum - microbiology
Tuberculosis
Tuberculosis, Pulmonary - drug therapy
Tuberculosis, Pulmonary - microbiology
Young Adult
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Summary:Background. Cameroon this last decade continues to present a low contribution of M. africanum and M. bovis in human tuberculosis (TB), while M. bovis was prevalent in cattle but all these pieces of information only concerned West and Center regions. Methods. We carried out the first study in Adamaoua, one of the most rearing regions of Cameroon, on the genetic structure and drug susceptibility of the MTBC strains isolated from newly diagnosed sputum smear-positive patients aged 15 years and above. For that purpose, spoligotyping, a modified 15 standard MIRU/VNTR loci typing, and the proportion method were used. Results. Four hundred and thirty-seven MTBC isolates were analyzed by spoligotyping. Of these, 423 were identified as M. tuberculosis, within the Cameroon family being dominant with 278 (65.7%) isolates; twelve (2.75%) isolates were classified as M. africanum and two as M. bovis. MIRU/VNTR typing of the most prevalent sublineage (SIT 61) suggested that this lineage is not a unique clone as thought earlier but could constitute a group of strains implicated to different pocket of TB transmission. Only M. tuberculosis sublineages were associated with antituberculosis drug resistance. Conclusion. These results showed the weak contribution of M. africanum and M. bovis to human active pulmonary tuberculosis in Cameroon even in the rearing region.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/2904832