Coated protein nanoclusters from influenza H7N9 HA are highly immunogenic and induce robust protective immunity

Abstract Recurring influenza viruses pose an annual threat to public health. A time-saving, cost-effective and egg-independent influenza vaccine approach is important particularly when responding to an emerging pandemic. We fabricated coated, two-layer protein nanoclusters from recombinant trimeric...

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Bibliographic Details
Published in:Nanomedicine 2017-01, Vol.13 (1), p.253-262
Main Authors: Wang, Li, Chang, Timothy Z, He, Yuan, Kim, Jong R, Wang, Shelly, Mohan, Teena, Berman, Zachary, Tompkins, S. Mark, Tripp, Ralph A, Compans, Richard W, Champion, Julie A, Wang, Bao-Zhong
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Enzyme-Linked Immunosorbent Assay
Female
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Immunity, Humoral
Influenza A Virus, H7N9 Subtype
Influenza vaccine
Influenza Vaccines - immunology
Internal Medicine
Mice
Mice, Inbred BALB C
Nanoparticles - chemistry
Neutralization Tests
Orthomyxoviridae Infections - prevention & control
Pandemic influenza
Protein nanoclusters
Recombinant Proteins - immunology
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Summary:Abstract Recurring influenza viruses pose an annual threat to public health. A time-saving, cost-effective and egg-independent influenza vaccine approach is important particularly when responding to an emerging pandemic. We fabricated coated, two-layer protein nanoclusters from recombinant trimeric hemagglutinin from an avian-origin H7N9 influenza A virus as an approach for vaccine development in response to an emerging pandemic. Assessment of the virus-specific immune responses and protective efficacy in mice immunized with the nanoclusters demonstrated that the vaccine candidates were highly immunogenic, able to induce protective immunity and long-lasting humoral antibody responses to this virus without the use of adjuvants. Because the advantages of the highly immunogenic coated nanoclusters also include rapid productions in an egg-independent system, this approach has great potential for influenza vaccine production not only in response to an emerging pandemic, but also as a replacement for conventional seasonal influenza vaccines.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2016.09.001