Simultaneous Biodegradation of Polyaromatic Hydrocarbons by a Stenotrophomonas sp: Characterization of nid Genes and Effect of Surfactants on Degradation

A polyaromatic hydrocarbon degrading bacterium was isolated from a petroleum contaminated site and designated as Stenotrophomonas sp. strain IITR87. It was found to utilize pyrene, phenanthrene and benzo(a)pyrene as sole carbon source, but not anthracene, chrysene and fluoranthene. Gas chromatograph...

Full description

Saved in:
Bibliographic Details
Published in:Indian journal of microbiology 2017-03, Vol.57 (1), p.60-67
Main Authors: Kumari, Smita, Regar, Raj Kumar, Bajaj, Abhay, Ch, Ratnasekhar, Satyanarayana, Gubbala Naga Venkata, Mudiam, Mohana Krishna Reddy, Manickam, Natesan
Format: Article
Language:English
Subjects:
Anthracene
Bacteria
Bioavailability
Biodegradation
Biomedical and Life Sciences
Carbon sources
Chromatography
Enzymes
Gas chromatography
Genes
Hydrocarbons
Life Sciences
Mass spectroscopy
Medical Microbiology
Metabolites
Microbiology
Mycobacterium vanbaalenii
Naphthalene
Original
Original Article
Oxidation
Petroleum hydrocarbons
Phenanthrene
Pollutants
Polycyclic aromatic hydrocarbons
Pyrene
Stenotrophomonas
Surfactants
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A polyaromatic hydrocarbon degrading bacterium was isolated from a petroleum contaminated site and designated as Stenotrophomonas sp. strain IITR87. It was found to utilize pyrene, phenanthrene and benzo(a)pyrene as sole carbon source, but not anthracene, chrysene and fluoranthene. Gas chromatography and mass spectroscopy analysis resulted in identification of pyrene metabolites namely monohydroxypyrene, 4-oxa-pyrene-5-one, dimethoxypyrene and monohydroxyphenanthrene. Southern hybridization using naphthalene dioxygenase gene ( nid A) as probe against the DNA of strain IITR87 revealed the presence of nid A gene. PCR analysis suggests dispersed occurrence of nid genes in the genome instead of a cluster as reported in a PAH-degrading Mycobacterium vanbaalenii PYR-1. The nid genes in strain IITR87, dioxygenase large subunit ( nid A), naphthalene dioxygenase small subunit ( nid B) and aldehyde dehydrogenase gene ( nid D) showed more than 97 % identity to the reported nid genes from Mycobacterium vanbaalenii PYR-1. Most significantly, the biodegradation of PAHs was enhanced 25–60 % in the presence of surfactants rhamnolipid and Triton X-100 due to increased solubilization and bioavailability. These results could be useful for the improved biodegradation of high-molecular-weight PAHs in contaminated habitats.
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-016-0612-6