Growth rate control of flagellar assembly in Escherichia coli strain RP437

The flagellum is a rotary motor that enables bacteria to swim in liquids and swarm over surfaces. Numerous global regulators control flagellar assembly in response to cellular and environmental factors. Previous studies have also shown that flagellar assembly is affected by the growth-rate of the ce...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-01, Vol.7 (1), p.41189-41189, Article 41189
Main Authors: Sim, Martin, Koirala, Santosh, Picton, David, Strahl, Henrik, Hoskisson, Paul A., Rao, Christopher V., Gillespie, Colin S., Aldridge, Phillip D.
Format: Article
Language:English
Subjects:
38
38/35
631/326
631/326/325/1506
Bacterial proteins
Crystal structure
E coli
Environmental factors
Flagella
Growth conditions
Growth rate
Humanities and Social Sciences
Motor task performance
multidisciplinary
Science
Science (multidisciplinary)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The flagellum is a rotary motor that enables bacteria to swim in liquids and swarm over surfaces. Numerous global regulators control flagellar assembly in response to cellular and environmental factors. Previous studies have also shown that flagellar assembly is affected by the growth-rate of the cell. However, a systematic study has not yet been described under controlled growth conditions. Here, we investigated the effect of growth rate on flagellar assembly in Escherichia coli using steady-state chemostat cultures where we could precisely control the cell growth-rate. Our results demonstrate that flagellar abundance correlates with growth rate, where faster growing cells produce more flagella. They also demonstrate that this growth-rate dependent control occurs through the expression of the flagellar master regulator, FlhD 4 C 2 . Collectively, our results demonstrate that motility is intimately coupled to the growth-rate of the cell.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep41189