Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR

• Published in: Saudi journal of biological sciences 2017-02, Vol.24 (2), p.367-370
• Main Authors: Al-Maary, Khalid S., Dawoud, Turki M., Mubarak, Ayman S., Hessain, Ashgan M., Galal, Hussein M., Kabli, Saleh A., Mohamed, Moussa I.
• Format: Article
• Language: English
• Subjects: Capsular antigens; Microbiological techniques; Molecular typing; Multiplex PCR; Original; P. multocida; التقنيات الميكروبيولوجية; باستوريلا مولتوسيدا; علم الأحياء الجزيئي
• ISSN: 1319-562X; 2213-7106
• DOI: 10.1016/j.sjbs.2016.06.006

The use of molecular techniques for detection and characterization of the Pasteurella multocida is very important for rapid and specific detection and characterization of the organism. During the period from 15th February, 2014 to 15th April, 2015, 425 nasopharyngeal swabs and 175 lung and spleen samples were collected and examined by conventional methods, 80 strains (18.82%) of P. multocida were isolated from the calves, sheep and goat with respiratory manifestation. Meanwhile, 77 strains (44%) were isolated from emergency slaughtered animals. All the recovered strains were positive for specific PCR for detection of P. multocida strains previously identified as P. multocida by standard microbiological techniques. Multiplex PCR for molecular typing of the capsular antigens of the recovered P. multocida revealed positive amplification of 1044bp fragments specific to the capsular antigen type A with 105 strains (66.88%), and amplification 511bp fragments of the capsular antigen type E with 52 strain (33.12%) and absence of B, D and F antigens. Multiplex PCR for molecular typing of the capsular antigens of P. multocida can be used as a simple, sensitive, rapid, reliable technique instead of the serological techniques for identification of the capsular antigens of P. multocida