Identification of an allatostatin from the tobacco hornworm Manduca sexta

A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of th...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-11, Vol.88 (21), p.9458-9462
Main Authors: Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA), Toschi, A, Miller, C.A, Kataoka, H, Quistad, G.B, Li, J.P, Carney, R.L, Schooley, D.A
Format: Article
Language:English
Subjects:
Amides
Amino Acid Sequence
Amino acids
Amino Acids - analysis
Animals
ANTAGONISTAS DE LAS HORMONAS
ANTAGONISTE D'HORMONE
Biochemistry. Physiology. Immunology
Biological and medical sciences
Biological Assay
Biosynthesis
Corpora allata
Female animals
Flow velocity
Fundamental and applied biological sciences. Psychology
HORMONAS
HORMONAS JUVENILES
HORMONAS SINTETICAS
HORMONE
HORMONE JUVENILE
HORMONE SYNTHETIQUE
Hormones
Insect biochemistry
Insect larvae
Insect pests
Insect Proteins
Insecta
Invertebrates
juvenile hormones
Juvenile Hormones - biosynthesis
Lepidoptera
MANDUCA SEXTA
Molecular Sequence Data
Moths - chemistry
PEPTIDE
Peptides - chemistry
Peptides - isolation & purification
PEPTIDOS
Physiology. Development
Pyrrolidonecarboxylic Acid - analogs & derivatives
SISTEMA NERVIOSO
Sphingidae
SYSTEME NERVEUX
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Description
Summary:A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of this 15-residue peptide has been determined: pGlu-Val-Arg-Phe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-OH, (where pGlu is pyroglutamate). To our knowledge, this neurohormone has no sequence similarity with any known neuropeptide from other organisms. The synthetic free acid and amide forms showed in vitro activity indistinguishable from that of native M. sexta allatostatin. The ED50 of synthetic M. sexta allatostatin on early fifth stadium larval corpora allata in vitro was approximately 2 nM. This inhibition was reversible. In a cross-species study, M. sexta allatostatin also inhibited the corpora allata of adult female Heliothis virescens but had no effect on the activity of corpora allata of adult females of the beetle Tenebrio molitor, the grasshopper Melanoplus sanguinipes, or the cockroach Periplaneta americana
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.21.9458