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Identification of an allatostatin from the tobacco hornworm Manduca sexta
A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of th...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1991-11, Vol.88 (21), p.9458-9462 |
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| container_end_page | 9462 |
| container_issue | 21 |
| container_start_page | 9458 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 88 |
| creator | Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA) Toschi, A Miller, C.A Kataoka, H Quistad, G.B Li, J.P Carney, R.L Schooley, D.A |
| description | A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of this 15-residue peptide has been determined: pGlu-Val-Arg-Phe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-OH, (where pGlu is pyroglutamate). To our knowledge, this neurohormone has no sequence similarity with any known neuropeptide from other organisms. The synthetic free acid and amide forms showed in vitro activity indistinguishable from that of native M. sexta allatostatin. The ED50 of synthetic M. sexta allatostatin on early fifth stadium larval corpora allata in vitro was approximately 2 nM. This inhibition was reversible. In a cross-species study, M. sexta allatostatin also inhibited the corpora allata of adult female Heliothis virescens but had no effect on the activity of corpora allata of adult females of the beetle Tenebrio molitor, the grasshopper Melanoplus sanguinipes, or the cockroach Periplaneta americana |
| doi_str_mv | 10.1073/pnas.88.21.9458 |
| format | article |
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(Sandoz Crop Protection, Palo Alto, CA) ; Toschi, A ; Miller, C.A ; Kataoka, H ; Quistad, G.B ; Li, J.P ; Carney, R.L ; Schooley, D.A</creator><creatorcontrib>Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA) ; Toschi, A ; Miller, C.A ; Kataoka, H ; Quistad, G.B ; Li, J.P ; Carney, R.L ; Schooley, D.A</creatorcontrib><description>A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of this 15-residue peptide has been determined: pGlu-Val-Arg-Phe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-OH, (where pGlu is pyroglutamate). To our knowledge, this neurohormone has no sequence similarity with any known neuropeptide from other organisms. The synthetic free acid and amide forms showed in vitro activity indistinguishable from that of native M. sexta allatostatin. The ED50 of synthetic M. sexta allatostatin on early fifth stadium larval corpora allata in vitro was approximately 2 nM. This inhibition was reversible. In a cross-species study, M. sexta allatostatin also inhibited the corpora allata of adult female Heliothis virescens but had no effect on the activity of corpora allata of adult females of the beetle Tenebrio molitor, the grasshopper Melanoplus sanguinipes, or the cockroach Periplaneta americana</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.21.9458</identifier><identifier>PMID: 1946359</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amides ; Amino Acid Sequence ; Amino acids ; Amino Acids - analysis ; Animals ; ANTAGONISTAS DE LAS HORMONAS ; ANTAGONISTE D'HORMONE ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Biological Assay ; Biosynthesis ; Corpora allata ; Female animals ; Flow velocity ; Fundamental and applied biological sciences. Psychology ; HORMONAS ; HORMONAS JUVENILES ; HORMONAS SINTETICAS ; HORMONE ; HORMONE JUVENILE ; HORMONE SYNTHETIQUE ; Hormones ; Insect biochemistry ; Insect larvae ; Insect pests ; Insect Proteins ; Insecta ; Invertebrates ; juvenile hormones ; Juvenile Hormones - biosynthesis ; Lepidoptera ; MANDUCA SEXTA ; Molecular Sequence Data ; Moths - chemistry ; PEPTIDE ; Peptides - chemistry ; Peptides - isolation & purification ; PEPTIDOS ; Physiology. Development ; Pyrrolidonecarboxylic Acid - analogs & derivatives ; SISTEMA NERVIOSO ; Sphingidae ; SYSTEME NERVEUX</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-11, Vol.88 (21), p.9458-9462</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-3ae528df16ad42ff9c8dfc0c4fc231fea67bc4c19da6c01c19314f419fd815093</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/21.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2357822$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2357822$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774,58219,58452</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5046151$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1946359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA)</creatorcontrib><creatorcontrib>Toschi, A</creatorcontrib><creatorcontrib>Miller, C.A</creatorcontrib><creatorcontrib>Kataoka, H</creatorcontrib><creatorcontrib>Quistad, G.B</creatorcontrib><creatorcontrib>Li, J.P</creatorcontrib><creatorcontrib>Carney, R.L</creatorcontrib><creatorcontrib>Schooley, D.A</creatorcontrib><title>Identification of an allatostatin from the tobacco hornworm Manduca sexta</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of this 15-residue peptide has been determined: pGlu-Val-Arg-Phe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-OH, (where pGlu is pyroglutamate). To our knowledge, this neurohormone has no sequence similarity with any known neuropeptide from other organisms. The synthetic free acid and amide forms showed in vitro activity indistinguishable from that of native M. sexta allatostatin. The ED50 of synthetic M. sexta allatostatin on early fifth stadium larval corpora allata in vitro was approximately 2 nM. This inhibition was reversible. In a cross-species study, M. sexta allatostatin also inhibited the corpora allata of adult female Heliothis virescens but had no effect on the activity of corpora allata of adult females of the beetle Tenebrio molitor, the grasshopper Melanoplus sanguinipes, or the cockroach Periplaneta americana</description><subject>Amides</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>ANTAGONISTAS DE LAS HORMONAS</subject><subject>ANTAGONISTE D'HORMONE</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Biosynthesis</subject><subject>Corpora allata</subject><subject>Female animals</subject><subject>Flow velocity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HORMONAS</subject><subject>HORMONAS JUVENILES</subject><subject>HORMONAS SINTETICAS</subject><subject>HORMONE</subject><subject>HORMONE JUVENILE</subject><subject>HORMONE SYNTHETIQUE</subject><subject>Hormones</subject><subject>Insect biochemistry</subject><subject>Insect larvae</subject><subject>Insect pests</subject><subject>Insect Proteins</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>juvenile hormones</subject><subject>Juvenile Hormones - biosynthesis</subject><subject>Lepidoptera</subject><subject>MANDUCA SEXTA</subject><subject>Molecular Sequence Data</subject><subject>Moths - chemistry</subject><subject>PEPTIDE</subject><subject>Peptides - chemistry</subject><subject>Peptides - isolation & purification</subject><subject>PEPTIDOS</subject><subject>Physiology. Development</subject><subject>Pyrrolidonecarboxylic Acid - analogs & derivatives</subject><subject>SISTEMA NERVIOSO</subject><subject>Sphingidae</subject><subject>SYSTEME NERVEUX</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAUhS0EKkNhjYQAZYFglanfsSU2qOIxUhEL6Nq649idVEk82A4t_x5HGQa6gZUf57vX5_og9JTgNcENO9uPkNZKrSlZay7UPbQiWJNaco3voxXGtKkVp_whepTSNcZYC4VP0AnRXDKhV2izad2YO99ZyF0Yq-ArGCvoe8gh5XI3Vj6Goco7V-WwBWtDtQtxvAlxqD7D2E4WquRuMzxGDzz0yT05rKfo8sP7b-ef6osvHzfn7y5qK3GTawZOUNV6IqHl1Htty8Fiy72ljHgHstlaboluQVpMyoYR7jnRvlVEYM1O0dul737aDq61xX6E3uxjN0D8aQJ05q4ydjtzFX4YQRvWlPLXh_IYvk8uZTN0yboy8OjClExDuaQak_-CROLys4IX8GwBbQwpReePXgg2c0hmDskoZSgxc0il4sXfI_zhl1SK_uqgQ7LQ-wij7dIRE5hLImaHLw_Y3P-3euedN_8EjJ_6PpfwCvl8Ia9TDvGIUiYaRWmRny2yh2DgKhYzl181aRiRnP0C52vGAw</recordid><startdate>19911101</startdate><enddate>19911101</enddate><creator>Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA)</creator><creator>Toschi, A</creator><creator>Miller, C.A</creator><creator>Kataoka, H</creator><creator>Quistad, G.B</creator><creator>Li, J.P</creator><creator>Carney, R.L</creator><creator>Schooley, D.A</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19911101</creationdate><title>Identification of an allatostatin from the tobacco hornworm Manduca sexta</title><author>Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA) ; Toschi, A ; Miller, C.A ; Kataoka, H ; Quistad, G.B ; Li, J.P ; Carney, R.L ; Schooley, D.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-3ae528df16ad42ff9c8dfc0c4fc231fea67bc4c19da6c01c19314f419fd815093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amides</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>ANTAGONISTAS DE LAS HORMONAS</topic><topic>ANTAGONISTE D'HORMONE</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Biosynthesis</topic><topic>Corpora allata</topic><topic>Female animals</topic><topic>Flow velocity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HORMONAS</topic><topic>HORMONAS JUVENILES</topic><topic>HORMONAS SINTETICAS</topic><topic>HORMONE</topic><topic>HORMONE JUVENILE</topic><topic>HORMONE SYNTHETIQUE</topic><topic>Hormones</topic><topic>Insect biochemistry</topic><topic>Insect larvae</topic><topic>Insect pests</topic><topic>Insect Proteins</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>juvenile hormones</topic><topic>Juvenile Hormones - biosynthesis</topic><topic>Lepidoptera</topic><topic>MANDUCA SEXTA</topic><topic>Molecular Sequence Data</topic><topic>Moths - chemistry</topic><topic>PEPTIDE</topic><topic>Peptides - chemistry</topic><topic>Peptides - isolation & purification</topic><topic>PEPTIDOS</topic><topic>Physiology. Development</topic><topic>Pyrrolidonecarboxylic Acid - analogs & derivatives</topic><topic>SISTEMA NERVIOSO</topic><topic>Sphingidae</topic><topic>SYSTEME NERVEUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA)</creatorcontrib><creatorcontrib>Toschi, A</creatorcontrib><creatorcontrib>Miller, C.A</creatorcontrib><creatorcontrib>Kataoka, H</creatorcontrib><creatorcontrib>Quistad, G.B</creatorcontrib><creatorcontrib>Li, J.P</creatorcontrib><creatorcontrib>Carney, R.L</creatorcontrib><creatorcontrib>Schooley, D.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kramer, S.J. (Sandoz Crop Protection, Palo Alto, CA)</au><au>Toschi, A</au><au>Miller, C.A</au><au>Kataoka, H</au><au>Quistad, G.B</au><au>Li, J.P</au><au>Carney, R.L</au><au>Schooley, D.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an allatostatin from the tobacco hornworm Manduca sexta</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-11-01</date><risdate>1991</risdate><volume>88</volume><issue>21</issue><spage>9458</spage><epage>9462</epage><pages>9458-9462</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A peptide (Manduca sexta allatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of this 15-residue peptide has been determined: pGlu-Val-Arg-Phe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-OH, (where pGlu is pyroglutamate). To our knowledge, this neurohormone has no sequence similarity with any known neuropeptide from other organisms. The synthetic free acid and amide forms showed in vitro activity indistinguishable from that of native M. sexta allatostatin. The ED50 of synthetic M. sexta allatostatin on early fifth stadium larval corpora allata in vitro was approximately 2 nM. This inhibition was reversible. In a cross-species study, M. sexta allatostatin also inhibited the corpora allata of adult female Heliothis virescens but had no effect on the activity of corpora allata of adult females of the beetle Tenebrio molitor, the grasshopper Melanoplus sanguinipes, or the cockroach Periplaneta americana</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1946359</pmid><doi>10.1073/pnas.88.21.9458</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-11, Vol.88 (21), p.9458-9462 |
| issn | 0027-8424 1091-6490 |
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| source | JSTOR Archival Journals and Primary Sources Collection; PubMed Central |
| subjects | Amides Amino Acid Sequence Amino acids Amino Acids - analysis Animals ANTAGONISTAS DE LAS HORMONAS ANTAGONISTE D'HORMONE Biochemistry. Physiology. Immunology Biological and medical sciences Biological Assay Biosynthesis Corpora allata Female animals Flow velocity Fundamental and applied biological sciences. Psychology HORMONAS HORMONAS JUVENILES HORMONAS SINTETICAS HORMONE HORMONE JUVENILE HORMONE SYNTHETIQUE Hormones Insect biochemistry Insect larvae Insect pests Insect Proteins Insecta Invertebrates juvenile hormones Juvenile Hormones - biosynthesis Lepidoptera MANDUCA SEXTA Molecular Sequence Data Moths - chemistry PEPTIDE Peptides - chemistry Peptides - isolation & purification PEPTIDOS Physiology. Development Pyrrolidonecarboxylic Acid - analogs & derivatives SISTEMA NERVIOSO Sphingidae SYSTEME NERVEUX |
| title | Identification of an allatostatin from the tobacco hornworm Manduca sexta |
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