DNA damage induced by Strontium-90 exposure at low concentrations in mesenchymal stromal cells: the functional consequences

• Published in: Scientific reports 2017-01, Vol.7 (1), p.41580-41580, Article 41580
• Main Authors: Musilli, S., Nicolas, N., El Ali, Z., Orellana-Moreno, P., Grand, C., Tack, K., Kerdine-Römer, S., Bertho, J. M.
• Format: Article
• Language: English
• Subjects: 13; 13/2; 13/31; 13/51; 631/80/304; 631/80/509; 631/80/82; 82/79; 82/80; 96/106; Accidents; Analysis of Variance; Animals; Biological effects; Bone marrow; Cell Death; Cell Differentiation - drug effects; Cell lines; Cell Proliferation - drug effects; Cellular Senescence - drug effects; Chronic exposure; Cytokines - metabolism; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded - drug effects; DNA damage; DNA Damage - drug effects; DNA Repair; Exposure; Hemopoiesis; Histones - metabolism; Humanities and Social Sciences; Humans; Life Sciences; Mesenchymal stem cells; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; Mesenchyme; multidisciplinary; Nuclear accidents & safety; Oxidation-Reduction - drug effects; Radioisotopes; Rats; Reactive Oxygen Species - metabolism; Science; Science (multidisciplinary); Senescence; Stromal cells; Strontium; Strontium Radioisotopes - pharmacology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep41580

90 Sr is one of the radionuclides released after nuclear accidents that can significantly impact human health in the long term. 90 Sr accumulates mostly in the bones of exposed populations. Previous research has shown that exposure induces changes in bone physiology both in humans and in mice. We hypothesize that, due to its close location with bone marrow stromal cells (BMSCs), 90 Sr could induce functional damage to stromal cells that may explain these biological effects due to chronic exposure to 90 Sr. The aim of this work was to verify this hypothesis through the use of an in vitro model of MS5 stromal cell lines exposed to 1 and 10 kBq.mL −1 of 90 Sr. Results indicated that a 30-minute exposure to 90 Sr induced double strand breaks in DNA, followed by DNA repair, senescence and differentiation. After 7 days of exposure, MS5 cells showed a decreased ability to proliferate, changes in cytokine expression, and changes in their ability to support hematopoietic progenitor proliferation and differentiation. These results demonstrate that chronic exposure to a low concentration of 90 Sr can induce functional changes in BMSCs that in turn may explain the health effects observed in following chronic 90 Sr exposure.