Domain organization of the yeast histone chaperone FACT: the conserved N-terminal domain of FACT subunit Spt16 mediates recovery from replication stress

The abundant nuclear complex termed FACT affects several DNA transactions in a chromatin context, including transcription, replication, and repair. Earlier studies of yeast FACT, which indicated the apparent dispensability of conserved sequences at the N terminus of the FACT subunit Cdc68/Spt16, pro...

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Bibliographic Details
Published in:Nucleic acids research 2004-01, Vol.32 (19), p.5894-5906
Main Authors: O'Donnell, A.F, Brewster, N.K, Kurniawan, J, Minard, L.V, Johnston, G.C, Singer, R.A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
binding proteins
Carrier Proteins - chemistry
Carrier Proteins - metabolism
Cell Cycle Proteins - chemistry
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
chromatin
Conserved Sequence
DNA Replication
faciliates chromatin transcription complex
histones
Histones - metabolism
Metazoa
Molecular Chaperones - chemistry
Mutation
phenotype
point mutation
Protein Structure, Tertiary
Protein Subunits - chemistry
Protein Subunits - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - chemistry
Saccharomyces cerevisiae Proteins - metabolism
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptional Elongation Factors
yeasts
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Summary:The abundant nuclear complex termed FACT affects several DNA transactions in a chromatin context, including transcription, replication, and repair. Earlier studies of yeast FACT, which indicated the apparent dispensability of conserved sequences at the N terminus of the FACT subunit Cdc68/Spt16, prompted genetic and biochemical studies reported here that suggest the domain organization for Spt16 and the other FACT subunit Pob3, the yeast homolog of the metazoan SSRP1 protein. Our findings suggest that each FACT subunit is a multidomain protein, and that FACT integrity depends on Pob3 interactions with the Spt16 Mid domain. The conserved Spt16 N-terminal domain (NTD) is shown to be without essential function during normal growth, but becomes important under conditions of replication stress. Genetic interactions suggest that some functions carried out by the Spt16 NTD may be partially redundant within FACT.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkh922