Regulation of ULK1 Expression and Autophagy by STAT1

• Published in: The Journal of biological chemistry 2017-02, Vol.292 (5), p.1899-1909
• Main Authors: Goldberg, Alexander A., Nkengfac, Bernard, Sanchez, Anthony M.J., Moroz, Nikolay, Qureshi, Salman T., Koromilas, Antonis E., Wang, Shuo, Burelle, Yan, Hussain, Sabah N., Kristof, Arnold S.
• Format: Article
• Language: English
• Subjects: Animals; apoptosis; autophagy; Autophagy - physiology; Autophagy-Related Protein-1 Homolog - biosynthesis; Autophagy-Related Protein-1 Homolog - genetics; Cell Line, Tumor; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Enzymologic - physiology; Humans; Intracellular Signaling Peptides and Proteins - biosynthesis; Intracellular Signaling Peptides and Proteins - genetics; Life Sciences; mammalian target of rapamycin (mTOR); Mice; Mice, Knockout; Promoter Regions, Genetic - physiology; proteolysis; signal transducers and activators of transcription 1 (STAT1); Signal Transduction; Sirolimus - pharmacology; STAT1 Transcription Factor - genetics; STAT1 Transcription Factor - metabolism
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M116.771584

Autophagy involves the lysosomal degradation of cytoplasmic contents for regeneration of anabolic substrates during nutritional or inflammatory stress. Its initiation occurs rapidly after inactivation of the protein kinase mammalian target of rapamycin (mTOR) (or mechanistic target of rapamycin), leading to dephosphorylation of Unc-51-like kinase 1 (ULK1) and autophagosome formation. Recent studies indicate that mTOR can, in parallel, regulate the activity of stress transcription factors, including signal transducer and activator of transcription-1 (STAT1). The current study addresses the role of STAT1 as a transcriptional suppressor of autophagy genes and autophagic activity. We show that STAT1-deficient human fibrosarcoma cells exhibited enhanced autophagic flux as well as its induction by pharmacological inhibition of mTOR. Consistent with enhanced autophagy initiation, ULK1 mRNA and protein levels were increased in STAT1-deficient cells. By chromatin immunoprecipitation, STAT1 bound a putative regulatory sequence in the ULK1 5′-flanking region, the mutation of which increased ULK1 promoter activity, and rendered it unresponsive to mTOR inhibition. Consistent with an anti-apoptotic effect of autophagy, rapamycin-induced apoptosis and cytotoxicity were blocked in STAT1-deficient cells but restored in cells simultaneously exposed to the autophagy inhibitor ammonium chloride. In vivo, skeletal muscle ULK1 mRNA and protein levels as well as autophagic flux were significantly enhanced in STAT1-deficient mice. These results demonstrate a novel mechanism by which STAT1 negatively regulates ULK1 expression and autophagy.