Network-based integration of systems genetics data reveals pathways associated with lignocellulosic biomass accumulation and processing

• Published in: Proceedings of the National Academy of Sciences - PNAS 2017-01, Vol.114 (5), p.1195-1200
• Main Authors: Mizrachi, Eshchar, Verbeke, Lieven, Christie, Nanette, Fierro, Ana C., Mansfield, Shawn D., Davis, Mark F., Gjersing, Erica, Tuskan, Gerald A., Van Montagu, Marc, Van de Peer, Yves, Marchal, Kathleen, Myburg, Alexander A.
• Format: Article
• Language: English
• Subjects: 09 BIOMASS FUELS; Adaptability; bioenergy; Biological Sciences; Biomass; Carbon - metabolism; cell wall; Cell Wall - metabolism; Chromosome Mapping; Crosses, Genetic; Eucalyptus; Eucalyptus - genetics; Eucalyptus - growth & development; Eucalyptus - metabolism; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes; Genes, Plant; Genetics; Hybridization, Genetic; Lignin - metabolism; lignocellulosic biomass; Metabolic Networks and Pathways - genetics; Metabolites; Models, Genetic; network-based data integration; Plant biomass; Plant Proteins - genetics; Plant Proteins - metabolism; Plantations; Quantitative Trait Loci; Renewable energy; systems genetics; Wood; Wood - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1620119114

As a consequence of their remarkable adaptability, fast growth, and superior wood properties, eucalypt tree plantations have emerged as key renewable feedstocks (over 20 million ha globally) for the production of pulp, paper, bioenergy, and other lignocellulosic products. However,most biomass properties such as growth, wood density, and wood chemistry are complex traits that are hard to improve in long-lived perennials. Systems genetics, a process of harnessing multiple levels of component trait information (e.g., transcript, protein, and metabolite variation) in populations that vary in complex traits, has proven effective for dissecting the genetics and biology of such traits. We have applied a network-based data integration (NBDI) method for a systems-level analysis of genes, processes and pathways underlying biomass and bioenergy-related traits using a segregating Eucalyptus hybrid population. We show that the integrative approach can link biologically meaningful sets of genes to complex traits and at the same time reveal the molecular basis of trait variation. Gene sets identified for related woody biomass traits were found to share regulatory loci, cluster in network neighborhoods, and exhibit enrichment for molecular functions such as xylan metabolism and cell wall development. These findings offer a framework for identifying the molecular underpinnings of complex biomass and bioprocessing-related traits. A more thorough understanding of the molecular basis of plant biomass traits should provide additional opportunities for the establishment of a sustainable bio-based economy.