Detailed Physical Map of Human Chromosomal Region 11q12-13 Shows High Meiotic Recombination Rate Around the MEN1 Locus

We have constructed a physical map of the region q12-13 on chromosome 11 by combining data generated from a panel of radiation-reduced somatic cell hybrids and pulsed-field gel electrophoresis (PFGE). Twenty different genetic markers have been sublocalized and ordered within this region and a total...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-12, Vol.88 (23), p.10609-10613
Main Authors: Janson, Marie, Larsson, Catharina, Werelius, Barbro, Jones, Carol, Glaser, Tom, Nakamura, Yusuke, Jones, C. Peter, Nordenskjold, Magnus
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
Cell lines
Chromosome Mapping
Chromosomes
Chromosomes, Human, Pair 11
Classical genetics, quantitative genetics, hybrids
Cricetinae
Crossing Over, Genetic
DNA
DNA Probes
Fundamental and applied biological sciences. Psychology
genes
Genetic Linkage
Genetic loci
Genetic mapping
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
Genomics
Human
Humans
Hybrid cells
Hybrid Cells - cytology
Hybrid Cells - physiology
Hybridity
Meiosis
Multiple Endocrine Neoplasia - genetics
recombination
Recombination, Genetic
Restriction Mapping
Somatic cells
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Summary:We have constructed a physical map of the region q12-13 on chromosome 11 by combining data generated from a panel of radiation-reduced somatic cell hybrids and pulsed-field gel electrophoresis (PFGE). Twenty different genetic markers have been sublocalized and ordered within this region and a total of 8.0 megabases has been mapped in detail using rare-cutting restriction endonucleases and PFGE. In two instances, the long-range restriction PFGE map spans the total distance between pairs of loci that have been previously mapped by genetic linkage in reference families. Comparison of this physical map with the available linkage map indicates a great variation in the recombination frequency over the region. The recombination rate is higher than expected, particularly for markers flanking the MEN1 region. Thus, for the closest pair of linked markers on the centromeric side, one centimorgan corresponds to ≈300 kilobases, and for markers on the telomeric side, one centimorgan corresponds to ≈350-600 kilobases.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.23.10609