Genetic and functional characterisation of the lactococcal P335 phage-host interactions

Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an indu...

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Bibliographic Details
Published in:BMC genomics 2017-02, Vol.18 (1), p.146-146, Article 146
Main Authors: Mahony, Jennifer, Oliveira, Joana, Collins, Barry, Hanemaaijer, Laurens, Lugli, Gabriele Andrea, Neve, Horst, Ventura, Marco, Kouwen, Thijs R, Cambillau, Christian, van Sinderen, Douwe
Format: Article
Language:English
Subjects:
Bacteriology
Bacteriophages
Bacteriophages - genetics
Bacteriophages - metabolism
Bacteriophages - physiology
Biochemistry, Molecular Biology
Calcium
Calcium - metabolism
Dairy industry
Divergence
Genes
Genetic Variation
Genomes
Genomic analysis
Genomics
Gram-positive bacteria
Host range
Host Specificity
Host Specificity - genetics
Lactococcus lactis
Lactococcus lactis - virology
Life Sciences
Microbiology and Parasitology
Morphology
Phages
Proteins
Viral Proteins
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
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Summary:Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context. The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called "baseplate", which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages while host range analysis highlighted a higher number of strains with CWPS type A (11 of 39 strains) are infected by the P335 phages assessed in this study than those with a C (five strains), B (three of 39 strains) or unknown (one of 39 strains) CWPS type. These analyses revealed significant divergence among RBP sequences, apparently reflecting their unique interactions with the host and particularly for strains with a type A CWPS. The implications of the genomic architecture of lactococcal P335 phages on serving as a general model for Siphoviridae phages are discussed.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-017-3537-5