Rapid analysis of components in Coptis chinensis Franch by ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry

Background: Coptis chinensis Franch is a traditional Chinese medical herb.Objective: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis F...

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Bibliographic Details
Published in:Pharmacognosy Magazine 2017-01, Vol.13 (49), p.175-179
Main Authors: Tian, Peng-peng, Zhang, Xiao-xu, Wang, Hong-ping, Li, Pu-ling, Liu, Yu-xin, Li, Shao-jing
Format: Article
Language:English
Subjects:
Acids
Chinese herbal medicine
Chinese medicine
Chromatography
Conflicts of interest
Gas flow
Ions
Liquid chromatography
Mass spectrometry
Organic acids
Original
Scientific imaging
Work stations
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Summary:Background: Coptis chinensis Franch is a traditional Chinese medical herb.Objective: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. Materials and Methods: Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18 column. The mobile phase consisted of 0.1% formic acid water (A) and 0.1% formic acid acetonitrile (B) with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight mass spectrometer, which was equipped with a Dual AJS ESI source operating in negative mode. Results: A total of 30 alkaloid and non-alkaloid components of Coptis chinensis Franch were identified in only 14 min.Conclusion: This study helped to provide a basis for the quality control of Coptis chinensis Franch. Abbreviations used: Q-TOF-MS: quadrupole time-of-flight mass spectrometry, UPLC: ultra-performance liquid chromatography, pos: positive, neg: negative.
ISSN:0973-1296
0976-4062
DOI:10.4103/0973-1296.197635