Long noncoding RNA CCAT1 acts as an oncogene and promotes chemoresistance in docetaxel-resistant lung adenocarcinoma cells

Chemoresistance remains one of the major obstacles in clinical treatment of lung adenocarcinoma (LAD). Indeed, docetaxel-resistant LAD cells present chemoresistance and epithelial-to-mesenchymal transition phenotypes. Long non-coding RNAs (lncRNAs) are known to promote tumorigenesis in many cancer t...

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Bibliographic Details
Published in:Oncotarget 2016-09, Vol.7 (38), p.62474-62489
Main Authors: Chen, Jing, Zhang, Kai, Song, Haizhu, Wang, Rui, Chu, Xiaoyuan, Chen, Longbang
Format: Article
Language:English
Subjects:
Adenocarcinoma - drug therapy
Adenocarcinoma - genetics
Animals
Apoptosis
bcl-X Protein - metabolism
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cell Survival
Drug Resistance, Neoplasm
Epithelial-Mesenchymal Transition
Female
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms - drug therapy
Lung Neoplasms - genetics
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs - metabolism
Neoplasm Invasiveness
Neoplasm Transplantation
Oncogenes
Phenotype
Research Paper
RNA, Long Noncoding - genetics
Taxoids - pharmacology
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Summary:Chemoresistance remains one of the major obstacles in clinical treatment of lung adenocarcinoma (LAD). Indeed, docetaxel-resistant LAD cells present chemoresistance and epithelial-to-mesenchymal transition phenotypes. Long non-coding RNAs (lncRNAs) are known to promote tumorigenesis in many cancer types. Here, we showed that the lncRNA colon cancer-associated transcript-1 (CCAT1) was upregulated in docetaxel-resistant LAD cells. Furthermore, downregulation of CCAT1 decreased chemoresistance, inhibited proliferation, enhanced apoptosis and reversed the epithelial-to-mesenchymal transition phenotype of docetaxel-resistant LAD cells. We also found that the oncogenic function of CCAT1 in docetaxel-resistant LAD cells depended on the sponging of let-7c. In turn, the sponging of let-7c by CCAT1 released Bcl-xl (a let-7c target), thereby promoting the acquisition of chemoresistance and epithelial-to-mesenchymal transition phenotypes in docetaxel-resistant LAD cells. Our data reveal a novel pathway underlying chemoresistance and the epithelial-to-mesenchymal transition in docetaxel-resistant LAD cells.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.11518