Chilling Affects Phytohormone and Post-Embryonic Development Pathways during Bud Break and Fruit Set in Apple (Malus domestica Borkh.)

• Published in: Scientific reports 2017-02, Vol.7 (1), p.42593-42593, Article 42593
• Main Authors: Kumar, Gulshan, Gupta, Khushboo, Pathania, Shivalika, Swarnkar, Mohit Kumar, Rattan, Usha Kumari, Singh, Gagandeep, Sharma, Ram Kumar, Singh, Anil Kumar
• Format: Article
• Language: English
• Subjects: 38/39; 38/77; 38/90; 631/449/1659; 631/449/1736; 631/449/2661; Abscisic acid; Apples; Chilling; Cold Temperature; Comparative analysis; Computational Biology - methods; Dormancy; Embryogenesis; Embryonic growth stage; Epigenesis, Genetic; Ethylene; Flowers - physiology; Fruit - physiology; Fruit set; Fruits; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Ontology; Gene Regulatory Networks; Gibberellic acid; Humanities and Social Sciences; Malus - physiology; Malus domestica; Molecular Sequence Annotation; multidisciplinary; Phytohormones; Plant Development; Plant Dormancy - genetics; Plant Growth Regulators - metabolism; Plant hormones; Ribonucleic acid; RNA; Science; Seasons; Signal Transduction; Transcription; Transcriptome
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep42593

The availability of sufficient chilling during bud dormancy plays an important role in the subsequent yield and quality of apple fruit, whereas, insufficient chilling availability negatively impacts the apple production. The transcriptome profiling during bud dormancy release and initial fruit set under low and high chill conditions was performed using RNA-seq. The comparative high number of differentially expressed genes during bud break and fruit set under high chill condition indicates that chilling availability was associated with transcriptional reorganization. The comparative analysis reveals the differential expression of genes involved in phytohormone metabolism, particularly for Abscisic acid, gibberellic acid, ethylene, auxin and cytokinin. The expression of Dormancy Associated MADS -box, Flowering Locus C -like, Flowering Locus T -like and Terminal Flower 1 -like genes was found to be modulated under differential chilling. The co-expression network analysis indentified two high chill specific modules that were found to be enriched for “post-embryonic development” GO terms. The network analysis also identified hub genes including Early flowering 7, RAF10, ZEP4 and F-box , which may be involved in regulating chilling-mediated dormancy release and fruit set. The results of transcriptome and co-expression network analysis indicate that chilling availability majorly regulates phytohormone-related pathways and post-embryonic development during bud break.