Visualisation of abscisic acid and 12-oxo-phytodienoic acid in immature Phaseolus vulgaris L. seeds using desorption electrospray ionisation-imaging mass spectrometry

The plant hormone abscisic acid (ABA) and the jasmonic acid related-compound 12-oxo-phytodienoic acid (OPDA) play crucial roles in seed development, dormancy, and germination. However, a lack of suitable techniques for visualising plant hormones has restricted the investigation of their biological m...

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Published in:Scientific reports 2017-02, Vol.7 (1), p.42977-42977, Article 42977
Main Authors: Enomoto, Hirofumi, Sensu, Takuya, Sato, Kei, Sato, Futoshi, Paxton, Thanai, Yumoto, Emi, Miyamoto, Koji, Asahina, Masashi, Yokota, Takao, Yamane, Hisakazu
Format: Article
Language:English
Subjects:
101/58
631/449/1741
639/638/11/296
Abscisic acid
Beans
Desorption
Dormancy
Germination
Hormones
Humanities and Social Sciences
Ions
Jasmonic acid
Liquid chromatography
Mass spectra
Mass spectrometry
Mass spectroscopy
Metabolites
multidisciplinary
Plant hormones
Science
Scientific imaging
Seed coats
Seeds
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Summary:The plant hormone abscisic acid (ABA) and the jasmonic acid related-compound 12-oxo-phytodienoic acid (OPDA) play crucial roles in seed development, dormancy, and germination. However, a lack of suitable techniques for visualising plant hormones has restricted the investigation of their biological mechanisms. In the present study, desorption electrospray ionisation-imaging mass spectrometry (DESI-IMS), a powerful tool for visualising metabolites in biological tissues, was used to visualise ABA and OPDA in immature Phaseolus vulgaris L. seed sections. The mass spectra, peak values and chemical formulae obtained from the analysis of seed sections were consistent with those determined for ABA and OPDA standards, as were the precursor and major fragment ions observed in tandem mass spectrometry (MS/MS) imaging. Furthermore, the precursor and fragment ion images showed similar distribution patterns. In addition, the localisation of ABA and OPDA using DESI-IMS was confirmed using liquid chromatography-MS/MS (LC-MS/MS). The results indicated that ABA was mainly distributed in the radical and cotyledon of the embryo, whereas OPDA was distributed exclusively in external structures, such as the hilum and seed coat. The present study is the first to report the visualisation of plant hormones using IMS, and demonstrates that DESI-IMS is a promising technique for future plant hormone research.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep42977