Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device

Efficient separations of particles with micron and submicron dimensions are extremely useful in preparation and analysis of materials for nanotechnological and biological applications. Here, we demonstrate a nonintuitive, yet efficient, separation mechanism for μm and subμm colloidal particles and o...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-06, Vol.88 (11), p.5920-5927
Main Authors: Luo, Jinghui, Muratore, Katherine A, Arriaga, Edgar A, Ros, Alexandra
Format: Article
Language:English
Subjects:
Animals
Arrays
Devices
Efficiency
Kinetics
Liver
Mathematical models
Mice
Microfluidic Analytical Techniques
Microfluidics
Migration
Mitochondria
Mitochondria, Liver - chemistry
Nanoparticles - chemistry
Nanostructure
Nanotechnology
Organelles
Organelles - chemistry
Particle Size
Proteins - chemistry
Proteins - isolation & purification
Rodents
Separation
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Summary:Efficient separations of particles with micron and submicron dimensions are extremely useful in preparation and analysis of materials for nanotechnological and biological applications. Here, we demonstrate a nonintuitive, yet efficient, separation mechanism for μm and subμm colloidal particles and organelles, taking advantage of particle transport in a nonlinear post array in a microfluidic device under the periodic action of electrokinetic and dielectrophoretic forces. We reveal regimes in which deterministic particle migration opposite to the average applied force occurs for a larger particle, a typical signature of deterministic absolute negative mobility (dANM), whereas normal response is obtained for smaller particles. The coexistence of dANM and normal migration was characterized and optimized in numerical modeling and subsequently implemented in a microfluidic device demonstrating at least 2 orders of magnitude higher migration speeds as compared to previous ANM systems. We also induce dANM for mouse liver mitochondria and envision that the separation mechanisms described here provide size selectivity required in future separations of organelles, nanoparticles, and protein nanocrystals.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b00837