Mechanistic Investigation on ROS Resistance of Phosphorothioated DNA

Phosphorothioated DNA (PT-DNA) exhibits a mild anti-oxidant property both in vivo and in vitro . It was found that 8-OHdG and ROS levels were significantly lower in dnd + (i.e. S + ) E. coli. , compared to a dnd − (i.e. S − ) strain. Furthermore, different from traditional antioxidants, phosphorothi...

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Published in:Scientific reports 2017-02, Vol.7 (1), p.42823-42823, Article 42823
Main Authors: Wu, Tingting, Huang, Qiang, Wang, Xiao-Lei, Shi, Ting, Bai, Linquan, Liang, Jingdan, Wang, Zhijun, Deng, Zixin, Zhao, Yi-Lei
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Language:English
Subjects:
132/122
631/92/56
8-Hydroxydeoxyguanosine
Antioxidants
Deoxyribonucleic acid
DNA
E coli
Genomes
Guanine
Humanities and Social Sciences
Hydroxyl radicals
Liquid chromatography
Mass spectrometry
Mass spectroscopy
multidisciplinary
Neutralization
Oxidants
Oxidation
Oxidizing agents
Phosphorothioate
Phosphorus
Science
Sulfur
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creator Wu, Tingting
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description Phosphorothioated DNA (PT-DNA) exhibits a mild anti-oxidant property both in vivo and in vitro . It was found that 8-OHdG and ROS levels were significantly lower in dnd + (i.e. S + ) E. coli. , compared to a dnd − (i.e. S − ) strain. Furthermore, different from traditional antioxidants, phosphorothioate compound presents an unexpectedly high capacity to quench hydroxyl radical. Oxidative product analysis by liquid chromatography-mass spectrometry and quantum mechanistic computation supported its unique anti-oxidant characteristic of the hydroxyl selectivity: phosphorothioate donates an electron to either hydroxyl radical or guanine radical derived from hydroxyl radical, leading to a PS • radical; a complex of PS • radical and OH − (i.e. the reductive product of hydroxyl radical) releases a highly reductive HS • radical, which scavenges more equivalents of oxidants in the way to high-covalent sulphur compounds such as sulphur, sulphite and sulphate. The PS-PO conversion (PS and PO denote phosphorus-sulphur and phosphorus-oxygen compounds, respectively) made a switch of extremely oxidative OH • to highly reductive HS • species, endowing PT-DNA with the observed high capacity in hydroxyl-radical neutralization. This plausible mechanism provides partial rationale as to why bacteria develop the resource-demanding PT modification on guanine-neighboring phosphates in genome.
doi_str_mv 10.1038/srep42823
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subjects 132/122
631/92/56
8-Hydroxydeoxyguanosine
Antioxidants
Deoxyribonucleic acid
DNA
E coli
Genomes
Guanine
Humanities and Social Sciences
Hydroxyl radicals
Liquid chromatography
Mass spectrometry
Mass spectroscopy
multidisciplinary
Neutralization
Oxidants
Oxidation
Oxidizing agents
Phosphorothioate
Phosphorus
Science
Sulfur
title Mechanistic Investigation on ROS Resistance of Phosphorothioated DNA
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