Structural characterization of EasH (Aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis

Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechan...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (99), p.14306-14309
Main Authors: Jakubczyk, Dorota, Caputi, Lorenzo, Stevenson, Clare E M, Lawson, David M, O'Connor, Sarah E
Format: Article
Language:English
Subjects:
Alkaloids
Aspergillus - enzymology
Aspergillus japonicus
Biocatalysis
Biosynthesis
Chemistry
Formations
Fungi
Indole Alkaloids - chemistry
Indole Alkaloids - metabolism
Models, Molecular
Organic compounds
Oxidase
Oxidation-Reduction
Oxidoreductases - chemistry
Oxidoreductases - metabolism
Protein Conformation
Structural analysis
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Summary:Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc08438a