Altered phosphatidylcholines expression in sputum for diagnosis of non-small cell lung cancer

Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality worldwide, and early diagnosis needs to be improved. We examined whether neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS) could be used to detect sputum lipids expression changes to enable e...

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Published in:Oncotarget 2016-09, Vol.7 (39), p.63158-63165
Main Authors: Zhang, Jianyong, Xu, Jianjun, Lu, Haiyan, Ding, Jianhua, Yu, Dongliang, Li, Penghui, Xiong, Jianwen, Liu, Xingxing, Chen, Huanwen, Wei, Yiping
Format: Article
Language:English
Subjects:
Adult
Aged
Carcinoma, Non-Small-Cell Lung - diagnosis
Case-Control Studies
Female
Humans
Lipids - chemistry
Lung Neoplasms - diagnosis
Male
Middle Aged
Phosphatidylcholines - chemistry
Phosphatidylglycerols - chemistry
Principal Component Analysis
Research Paper
Spectrometry, Mass, Electrospray Ionization
Sphingomyelins - chemistry
Sputum - chemistry
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Summary:Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality worldwide, and early diagnosis needs to be improved. We examined whether neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS) could be used to detect sputum lipids expression changes to enable earlier diagnosis. Overall, 167 NSCLC patients and 140 controls were enrolled. The main peaks in the sputum of patients with NSCLC patients differed from controls (83.3% of total variability), and the signals were not associated with pathological type, TNM stage or smoking history. The relative abundance of peaks at m/z734, m/ z756, m/z772, m/z782, m/z798 and m/z803 reliably distinguished NSCLC sputum from control. Collision-induced dissociation confirmed that m/z734, m/z756, and m/ z772 represented [DPPC + H]+, [DPPC + Na]+, and [DPPC + K]+, respectively, and m/ z782, m/z798, and m/z803 represented sphingomyelin, phosphatidylglycerol, and phosphatidylglycerolphosphate, respectively. The relative abundance of DPPC was clearly lower in NSCLC sputum than in control, and the relative abundances of phosphatidylglycerol and phosphatidylglycerolphosphate were higher in NSCLC sputum than in control. The detection of changes in sputum lipids with ND-EESI-MS may be a noninvasive, radiation-free, relatively inexpensive, repeatable, and efficient method for diagnosis of NSCLC.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.11283